LncRNA GAS5对结直肠癌5-FU耐药的作用研究  

Study on role of LncRNA GAS5 on 5-FU resistance in colorectal cancer

作　　者：聂启鸿 钟晓鸣[3] NIE Qi-hong;ZHONG Xiao-ming(Department of Oncology,Jiangxi Cancer Hospital,Nanchang 330029,China)

机构地区：[1]江西省肿瘤医院肿瘤科,330029 [2]赣州市人民医院肿瘤科,341000 [3]江西省肿瘤医院放疗科,330029

出　　处：《中国现代药物应用》2023年第10期177-180,共4页Chinese Journal of Modern Drug Application

摘　　要：目的 探讨长链非编码RNA生长停滞特异性转录本5(LncRNA GAS5)对结直肠癌5-氟尿嘧啶(5-FU)耐药的作用。方法 选取人结肠癌细胞系HCT-8和SW480,建立5-FU耐药细胞株,构建LncRNA GAS5过表达结直肠癌细胞模型。反转录-聚合酶链式反应(RT-PCR)检测LncRNA GAS5在结直肠癌5-FU耐药细胞系中表达;CCK-8检测细胞增殖;流式细胞术检测细胞凋亡。结果 RT-PCR检测显示,LncRNA GAS5在结直肠癌HCT-8细胞与SW480细胞中的表达水平低于正常结肠细胞,差异有统计学意义(P<0.05);LV5-GAS5组HCT-8细胞、SW480细胞中LncRNA GAS5表达高于Vector组,差异有统计学意义(P<0.05)。CCK-8实验检测显示,LncRNA GAS5能明显抑制HCT-8细胞和SW480细胞的增殖(P<0.05)。AnnexinV/PI双重染色及流式细胞术结果显示,在结直肠癌HCT-8细胞与SW480细胞中LV5-GAS5组的细胞凋亡比例高于Vector组,差异有统计学意义(P<0.05)。结论 LncRNA GAS5在结直肠癌5-FU耐药中发挥重要作用,能抑制结肠癌细胞系的增殖,并促进细胞凋亡。Objective To discuss the role of long non-coding RNA-growth arrest-specific 5(LncRNA GAS5)on 5-fluorouracil(5-FU)resistance in colorectal cancer.Methods Human colon cancer cell lines HCT-8 and SW480 were selected to establish 5-FU resistant cell lines,and LncRNA GAS5 overexpression colorectal cancer cell model was constructed.LncRNA GAS5 expression was detected by reverse transcription polymerase chain reaction(RT-PCR)in 5-FU resistant cell lines of colorectal cancer.CCK-8 was used to detect cell proliferation,and apoptosis was detected by flow cytometry.Results RT-PCR showed that the expression level of LncRNA GAS5 in colorectal cancer HCT-8 and SW480 cells was significantly lower than that in normal colon cells,and the difference was statistically significant(P<0.05).The expression of LncRNA GAS5 in HCT-8 cells and SW480 cells in LV5-GAS5 group was significantly higher than that in Vector group,and the difference was statistically significant(P<0.05).CCK-8 assay showed that LncRNA GAS5 could significantly inhibit the proliferation of HCT-8 cells and SW480 cells(P<0.05).AnnexinV/PI double staining and flow cytometry showed that the apoptosis ratio of LV5-GAS5 group in HCT-8 cells and SW480 cells was also significantly higher than that in vector group,and the difference was statistically significant(P<0.05).Conclusion LncRNA GAS5 plays an important role in the 5-FU resistance of colorectal cancer,which can inhibit the proliferation of colon cancer cell lines and promote cell apoptosis.

关 键 词：结直肠癌 长链非编码RNA生长停滞特异性转录本5 5-氟尿嘧啶 耐药 

分 类 号：R735.34[医药卫生—肿瘤]