中药渣饲喂对蚯蚓生长性能及消化酶活性的影响  

Effect of Traditional Chinese Medicine Residues On Growth Performance and Digestive Enzyme Activity of Earthworms

作　　者：李杨 康俊港 马占飞 王亚博 赵博伟 姜国均[1] LI Yang;KANG Jungang;MA Zhanfei;WANG Yabo;ZHAO Bowei;JIANG Guojun(College of Veterinary Medicine,Hebei Agricultural University,Baoding 071000,China;Hebei Provincial Animal Husbandry Station,Shijiazhuang 050033,China)

机构地区：[1]河北农业大学动物医学院,河北保定071000 [2]河北省畜牧总站,河北石家庄050033

出　　处：《河南农业科学》2023年第5期156-161,共6页Journal of Henan Agricultural Sciences

基　　金：河北省二期现代农业产业技术体系项目(HBCT2018130205)。

摘　　要：为解决畜禽粪污和药渣对环境污染的问题,探索中药渣和牛粪资源化利用的新途径,试验选取平均体质量为239.40 mg、平均体长为5.51 cm的健康蚯蚓,随机分成A、B、C和D组,每组设3个重复,在发酵牛粪的基础上分别添加0、10%、20%、30%的五味健脾颗粒药渣,室外饲养28 d。饲养0、28 d时,每组随机取蚯蚓9条,测量其体质量和体长;饲养0、7、14、21、28 d时每组随机取蚯蚓9条,提取蚯蚓粗酶液,用酶联免疫吸附法(ELISA)检测蛋白酶、纤维素酶和磷酸酶活性。饲养28 d时,与A组相比,C组蚯蚓体质量和体长显著增加,增重率提高55.50个百分点,体长增加1.83 cm;C组在饲养28 d时蛋白酶活性、纤维素酶活性和磷酸酶活性达到最高,且显著高于对照组(P<0.05)。以上结果表明,五味健脾颗粒药渣促进了蚯蚓生长发育,增强了消化酶活性,添加20%药渣效果最好。In order to solve the problem of environmental pollution caused by livestock and poultry feces and drug residues,and explore a new way of resource utilization of traditional Chinese medicine residues and cow dung,healthy earthworms with average body weight of 239.40 mg and average body length of5.51 cm were selected and randomly divided into groups A,B,C and D,with three replicates in each group.On the basis of fermented cow dung,0,10%,20% and 30% pharmaceutical residues of Wuwei Jianpi granules were added,respectively,and earthworms were fed outdoors for 28 days.On the 0th and28th days of feeding,9 earthworms were randomly selected from each group,and their body weight and body length were measured.At 0,7,14,21 and 28 days of feeding,9 earthworms were randomly selected from each group to extract crude enzyme solution,and enzyme linked immunosorbent assay was used to detect the activities of protease,cellulase and phosphatase.On the 28th day,compared with group A,the body weight and body length of earthworms in group C were significantly increased,the weight gain rate was increased by 55.50 percentage points,and the body length was increased by 1.83 cm.The activities of protease,cellulase and phosphatase in group C reached the highest(P<0.05)on the 28th day.The above results show that the drug residues of Wuwei Jianpi granules promote the growth and development of earthworms and enhance the digestive enzyme activities,and the best effect is obtained by adding 20%of the drug residues.

关 键 词：蚯蚓 中药渣 发酵牛粪 蛋白酶活性 纤维素酶活性 磷酸酶活性 

分 类 号：S855.3[农业科学—临床兽医学]