Proteomic and phosphoproteomic analysis reveal threonine deficiency increases hepatic lipid deposition in Pekin ducks via reducing STAT phosphorylation  

作　　者：Yong Jiang Zhong Zhuang Wenqia Jia Zhiguo Wen Ming Xie Hao Bai Yulin Bi Zhixiu Wang Guobin Chang Shuisheng Hou Guohong Chen 

机构地区：[1]College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China [2]State Key Laboratory of Animal Nutrition,Key Laboratory of Animal(Poultry)Genetics Breeding and Reproduction,Ministry of Agriculture and Rural Affairs,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China [3]Joint International Research Laboratory of Agriculture and Agri-Product Safety,the Ministry of Education of China,Institutes of Agricultural Science and Technology Development,Yangzhou University,Yangzhou 225009,China [4]Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs,Institute of Feed Research,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《Animal Nutrition》2023年第2期249-260,共12页动物营养（英文版）

基　　金：supported by National Natural Science Foundation of China(31902174);Natural Science Foundation of Jiangsu Province(BK20190902);Tackled key technologies in agriculture and rural areas of Jiangsu Science and Technology Plan(BE2022310).

摘　　要：Dietary threonine(Thr)deficiency enhances triglyceride(TG)deposition in the liver of Pekin ducks,which injures hepatic function and impairs growth performance.However,the underlying molecular mechanisms remain unclear.In the present study,we investigated the effects of dietary Thr deficiency on the expressions of proteins and phosphoproteins in liver of Pekin ducks,to identify the underlying molecular changes.A total of 300 one-day-old ducklings were divided into 3 groups with 10 replicates of 10 birds.All ducks were fed corn-wheat-peanut meal diets containing 0.46%,0.71%,and 0.96%Thr,respectively,from 1 to 21 days of age.Growth performance,serum parameters,hepatic TG content,and expression of genes involved in lipid metabolism of Pekin ducks were determined.A Thr deficiency group(Thr-D,0.46%Thr)and a Thr sufficiency group(Thr-S,0.71%Thr)were selected for subsequent proteomic and phosphoproteomic analysis.The results showed that Thr-D reduced the growth performance(P<0.001),and increased the plasma concentrations of cholesterol,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,and hepatic TG(P<0.05).Thr-D increased gene expression related to fatty acid and TG synthesis(P<0.05).A total of 176 proteins and 259 phosphosites(containing 198 phosphoproteins)were observed to be differentially expressed as a result of Thr-D.The upregulated proteins were enriched in the pathway related to amino acid metabolism,peroxisome.The down-regulated proteins were enriched in linolenic and arachidonic acid metabolism,and the Janus kinase-signal transducer and activator of transcription(JAK-STAT)signaling pathway.The upregulated phos-phoproteins were enriched in the pathways related to fatty acid biosynthesis,fructose and mannose metabolism,and glycolysis/gluconeogenesis.Thr-D reduced the phosphorylation of STAT1 at S729 and STAT3 at S728,and expression of STAT5B.In contrast,Thr-D increased non-receptor tyrosine-protein kinase(TYK2)expression and STAT1 phosphorylation at S649.Taken together,dietary Thr-D increa

关 键 词：Threonine deficiency Triglyceride accumulation PROTEOMIC Phosphoproteomic Pekin duck 

分 类 号：R834[医药卫生—航空、航天与航海医学]