The sperm-interacting proteome in the bovine isthmus and ampulla during the periovulatory period  

作　　者：Coline Mahe Regis Lavigne Emmanuelle Com Charles Pineau Aleksandra Maria Zlotkowska Guillaume Tsikis Pascal Mermillod Jennifer Schoen Marie Saint-Dizier 

机构地区：[1]CNRS,IFCE,INRAE,Universite de Tours,PRC,37380 Nouzilly,France [2]Univ Rennes,Inserm,EHESP,Irset(Institut de Recherche en Sante,Environnement Et Travail)-UMR-S 1085,F‑35000 Rennes,France [3]Univ Rennes,CNRS,Inserm,Biosit UAR 3480 US_S 018,Protim Core Facility,35000 Rennes,France [4]Institute of Reproductive Biology,Leibniz Institute for Farm Animal Biology,FBN,Dummerstorf,Germany [5]Present Address:Department of Reproduction Biology,Leibniz Institute for Zoo and Wildlife Research(IZW),Berlin,Germany

出　　处：《Journal of Animal Science and Biotechnology》2023年第3期980-1000,共21页畜牧与生物技术杂志（英文版）

基　　金：funded by INRAE and Agence Nationale de la Recherche under the grant number ANR-18-CE92-0049;supported by grants from Biogenouest;Infrastructures en Biologie Santéet Agronomie (IBiSA);Conseil Régional de Bretagne awarded to Protim proteomics core facility。

摘　　要：Background Spermatozoa interact with oviduct secretions before fertilization in vivo but the molecular players of this dialog and underlying dynamics remain largely unknown.Our objectives were to identify an exhaustive list of sperm-interacting proteins(SIPs)in the bovine oviduct fluid and to evaluate the impact of the oviduct anatomical region(isthmus vs.ampulla)and time relative to ovulation(pre-ovulatory vs.post-ovulatory)on SIPs number and abundance.Methods Pools of oviduct fluid(OF)from the pre-ovulatory ampulla,pre-ovulatory isthmus,post-ovulatory ampulla,and post-ovulatory isthmus in the side of ovulation were collected from the slaughterhouse.Frozen-thawed bull sperm were incubated with OF or phosphate-buffered saline(control)for 60 min at 38.5℃.After protein extraction and digestion,sperm and OF samples were analyzed by nanoLC-MS/MS and label-free protein quantification.Results A quantitative comparison between proteins identified in sperm and OF samples(2333 and 2471 proteins,respectively)allowed for the identification of 245 SIPs.The highest number(187)were found in the pre-ovulatory isthmus,i.e.,time and place of the sperm reservoir.In total,41 SIPs(17%)were differentially abundant between stages in a given region or between regions at a given stage and 76 SIPs(31%)were identified in only one region×stage condition.Functional analysis of SIPs predicted roles in cell response to stress,regulation of cell motility,fertilization,and early embryo development.Conclusion This study provides a comprehensive list of SIPs in the bovine oviduct and evidences dynamic spatiotemporal changes in sperm-oviduct interactions around ovulation time.Moreover,these data provide protein candidates to improve sperm conservation and in vitro fertilization media.

关 键 词：AMPULLA Fallopian tube INTERACTOME ISTHMUS OVIDUCT OVULATION Post-ovulatory Pre-ovulatory Proteomics SPERMATOZOA 

分 类 号：S814.1[农业科学—畜牧学]