Antimicrobial peptides act on the rumen the performance of castrated bulls  

作　　者：Jinping Shi Yu Lei Jianping Wu Zemin Li Xiao Zhang Li Jia Ying Wang Yue Ma Ke Zhang Qiang Cheng Zhao Zhang Yannan Ma Zhaomin Lei 

机构地区：[1]College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China [2]Key Laboratory of Animal Genetics,Breeding and Reproduction of Shanxi Province,College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China [3]Institute of Rural Development,Northwest Normal University,Lanzhou 730070,China [4]Jingchuan Xu Kang Food Co.,Ltd.,Pingliang 744300,China [5]Gansu Huarui Agriculture Co.,Ltd.,Zhangye 734500,China

出　　处：《Journal of Animal Science and Biotechnology》2023年第3期1218-1234,共17页畜牧与生物技术杂志（英文版）

基　　金：financially supported by Research and application of corn straw forage and beef cattle high-efficiency and quality production technology (Provincial Education Science and Technology Innovation Project) (GSSYLXM-02);the Gansu beef cattle quality fattening project (GSAXMLZ-2021-01);the Application of Pingliang Red Bull Planting and Breeding Combined with High-efficiency Circular Production System Construction Technology Application (2020C-08);the local funding (GSSLCSX-2020-1)。

摘　　要：Background Many countries have already banned the use of antibiotics in animal husbandry,making it extremely difficult to maintain animal health in livestock breeding.In the livestock industry,there is an urgent need to develop alternatives to antibiotics which will not lead to drug resistance on prolonged use.In this study,eighteen castrated bulls were randomly divided into two groups.The control group(CK)was fed the basal diet,while the antimicrobial peptide group(AP)was fed the basal diet supplemented with 8 g of antimicrobial peptides in the basal diet for the experimental period of 270 d.They were then slaughtered to measure production performance,and the ruminal contents were isolated for metagenomic and metabolome sequencing analysis.Result The results showed that antimicrobial peptides could improve the daily weight,carcass weight,and net meat weight of the experimental animals.Additionally,the rumen papillae diameter and the micropapillary density in the AP were significantly greater than those in the CK.Furthermore,the determination of digestive enzymes and fermentation parameters showed that the contents of protease,xylanase,andβ-glucoside in the AP were greater than those in the CK.However,lipase content in the CK was greater than that in the AP.Moreover,the content of acetate,propionate,butyrate,and valerate was found to be greater in AP than those in CK.The metagenomic analysis annotated 1993 differential microorganisms at the species level.The KEGG enrichment of these microorganisms revealed that the enrichment of drug resistance-related pathways was dramatically decreased in the AP,whereas the enrichment of immune-related pathways was significantly increased.There was also a significant reduction in the types of viruses in the AP.187 probiotics with significant differences were found,135 of which were higher in AP than in CK.It was also found that the antimicrobial mechanism of the antimicrobial peptides was quite specific.Seven low-abundance microorganisms(Acinetobactersp.Ac1271,Aequorivita soes

关 键 词：Antimicrobial peptides Castrated bull Growth performance METABOLITES MICROORGANISMS RUMEN 

分 类 号：S823.5[农业科学—畜牧学]