ARID1A在肝内胆管癌患者中的表达及与肿瘤微环境的关系  

ARID1A expression in intrahepatic cholangiocarcinoma and its relationship with tumor recurrence,metastasis and tumor-specific immunomarkers

作　　者：张超[1] 陈卓妙语 程倩 李照[1] 高杰[1] 朱继业[1] Zhang Chao;Chen Zhuomiaoyu;Cheng Qian;Li Zhao;Gao Jie;Zhu Jiye(Department of Hepatobiliary Surgery,Peking University People's Hospital,Beijing 100044,China)

机构地区：[1]北京大学人民医院肝胆外科,北京100044

出　　处：《中华普通外科杂志》2023年第5期362-366,共5页Chinese Journal of General Surgery

基　　金：国家自然科学基金(82172613);首都卫生发展科研专项基金(2022-2-4084)。

摘　　要：目的探讨AT丰富结合域(ARID1A)蛋白在肝内胆管癌(intrahepatic cholangiocarcinoma,ICC)患者肿瘤中的表达情况及其与患者肿瘤微环境的相关性。方法回顾性分析2015年1月至2021年5月在北京大学人民医院行肝切除术的110例ICC患者的临床病理及生存情况,使用免疫组化染色测定肿瘤组织中ARID1A、细胞程序性死亡配体1(programmed cell death 1 ligand 1,PD-L1)表达及微环境中程序性死亡受体1(programmed cell death protein 1,PD-1)、分化簇8(cluster of differentiation 8,CD8)表达情况。结果27例患者肿瘤组织不表达ARID1A,肿瘤不表达ARID1A蛋白与肿瘤高PD-L1表达,微环境中高PD-1、CD8表达具有明显相关性(P<0.05)。多因素分析显示ARID1A表达情况、术前CEA水平、术前CA19-9水平、是否合并淋巴结转移、肿瘤数目是患者术后生存的独立危险因素。结论肿瘤不表达ARID1A提示着ICC患者的不良预后,ARID1A缺失表达的ICC肿瘤微环境中PD-L1、PD-1、CD8蛋白高表达,提示这类患者是免疫治疗的潜在获益人群。Objective To investigate the expression level between AT-Rich Interaction Domain 1A(ARID1A)in intrahepatic cholangiocarcinoma(ICC)and the correlation with tumor microenvironment.Methods The clinicopathological and survival data of 110 ICC patients undergoing radical hepatectomy in Peking University People's Hospital from Jan 2015 to May 2021 were retrospectively analyzed.Immunohistochemical staining was used to detect the expressions of ARID1A,programmed cell death 1 ligand 1(PD-L1)in tumor tissues,programmed cell death protein 1(PD-1)and cluster of differentiation 8(CD8)in the microenvironment.The relationship between ARID1A expression and PD-L1,PD-1,CD8 protein expression was analyzed.Results Twenty seven patients did not express ARID1A,absence of ARID1A was associated with high PD-L1,PD-1 and CD8 expression(P<0.05).Multivariate analysis showed ARID1A expression,preoperative CEA level,preoperative CA19-9 level,lymph node metastasis and tumor number were independent risk factors.Conclusion Absent expression of ARID1A suggests poor prognosis of ICC patients,high expression of PD-L1,PD-1 and CD8 protein in ICC tumor microenvironment with ARID1A-deficient expression suggests a possible prognosis benefit by using anti-PD-1,anti-PD-L1 and other immunotherapy regimens.

关 键 词：蛋白质相互作用域和基序 胆管上皮癌 预后 肿瘤微环境 

分 类 号：R735.8[医药卫生—肿瘤]