Integrated Use of Alternative Methods for Evaluating the Skin Sensitization Potencies of Five Frequently Used Preservatives in Cosmetics  

作　　者：Wang Rui Wang Huan Han Yu-qing Zhao Jinfeng Yan Shiyu Pan Yao 

机构地区：[1]Department of Cosmetics,College of Chemistry and Materials Engineering,Beijing Technology and Business University,China [2]Beijing Key Laboratory of Plant Research and Development,China [3]Institute of Cosmetic Regulatory Science,Beijing Technology and Business University,China

出　　处：《China Detergent & Cosmetics》2023年第2期38-47,共10页日用化学品科学（英文版）

摘　　要：In vitro skin sensitization testing methods based on the adverse outcome pathway(AOP)were used to evaluate the skin sensitization potencies of 5 commonly used preservatives.According to the“2 out of 3”principle of the integrated approaches to testing and assessment(IATA)the direct peptide reactivity assay(DPRA)and the human cell line activation test(h-CLAT)were used to detect the preservatives commonly used in cosmetics,including phenoxyethanol.methyl paraben,propyl paraben,imidazolidinyl urea and DMDM hydantoin.The DPRA and the h-CLA were carried out according to the OEC442C and 442E guidelines,respectively.The results show that.phenoxyethanol and methyl paraben are both negative in DPRA and h-CLAT while imidazolidinyl urea and DMDM hydantoin are both positive in these two tests.Propyl paraben has negative result in DPRA but positive result in h-CLAT.Therefore,imidazolidiny urea and DMDM hydantoin are sensitizers,while phenoxyethanol and methylparaben are non-sensitizers.Taken animal and human data into consideration,it is predicted that propyl paraben should be a non-sensitizer.The combination of DPRA and h-CLAT can make up for the limitations of using a single method,and it is suitable for the preliminary screening of cosmetic raw materials according to skin sensitization.

关 键 词：Skin sensitization PRESERVATIVE alternative method DPRA h-CLAT AOP 

分 类 号：TQ658[化学工程—精细化工]