逐瘀清心胶囊对精神分裂症大鼠神经组织及髓鞘病变影响及机制  

Effect of Zhuyu Qingxin capsule on nerve tissue and myelin sheath lesions in schizophrenic rats and its mechanism

作　　者：彭爱能 赵玉萍 赵永厚 于明 柴剑波 PENG Aineng;ZHAO Yuping;ZHAO Yonghou;YU Ming;CHAI Jianbo(Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China;Heilongjiang Mental Hospital,Harbin 150036,Heilongjiang,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江神志医院,黑龙江哈尔滨150036

出　　处：《现代中西医结合杂志》2023年第7期890-894,908,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：黑龙江省中医药科研项目(ZHY2020-183,ZHY2022-050);国家自然科学基金项目(81873299,81973747);黑龙江省应用技术研究与开发计划项目(GZ16C001)。

摘　　要：目的 观察逐瘀清心胶囊对MK-801诱导的精神分裂症大鼠神经组织及髓鞘病变的影响,探讨逐瘀清心胶囊治疗精神分裂症的作用机制。方法 将40只雄性SD大鼠随机分为空白组、模型组、逐瘀清心组、利培酮组,每组10只。除空白组外,其余组大鼠应用MK-801药液腹腔注射构建精神分裂症模型。造模成功后,逐瘀清心组给予逐瘀清心胶囊混悬液0.054 g/(kg·d)灌胃,利培酮组给予利培酮混悬液0.036 mg/(kg·d)灌胃,空白组及模型组给予等剂量蒸馏水灌胃,均1次/d,连续灌胃28 d。HE染色观察大鼠脑组织病理形态,LFB染色观察脑白质髓鞘病变情况,Western blot法观察脑额叶皮质、海马、扣带组织中分化抑制因子2(Id2)、少突胶质细胞转录因子1(Olig1)、少突胶质细胞转录因子2(Olig2)蛋白表达情况。结果 模型组大鼠脑组织神经元细胞核固缩明显,髓鞘组织结构松散,存在明显的脱髓鞘病变;与模型组比较,逐瘀清心组、利培酮组细胞形态较为规则,核固缩现象减少,脱髓鞘病变减轻,结构较为致密,且逐瘀清心组改善更明显。与空白组比较,模型组大鼠额叶皮质、海马、扣带组织中Id2蛋白表达量均明显升高(P均<0.05),Olig1和Olig2蛋白表达量均明显降低(P均<0.05);与模型组比较,逐瘀清心组和利培酮组大鼠额叶皮质、海马、扣带组织中Id2蛋白表达量均明显降低(P均<0.05),Olig1和Olig2蛋白表达量均明显升高(P均<0.05)。结论 逐瘀清心胶囊可明显减轻精神分裂症大鼠神经组织损伤,其作用机制可能与调控脑额叶皮质、海马、扣带中Id2、Olig1、Olig2蛋白的表达,修复髓鞘病变有关。Objective It is to observe the effect of Zhuyu Qingxin capsule on nerve tissue and myelin sheath lesions in rats with schizophrenic induced by MK-801,and to explore the mechanism of Zhuyu Qingxin capsule in the treatment of schizophrenia.Methods Forty male SD rats were randomly divided into blank group,model group,Zhuyu Qingxin group,and risperidone group,with 10 rats in each group.Except for the blank group,the rats in the other groups were injected intraperitoneally with MK-801 solution to establish schizophrenia models.After successful modeling,the Zhuyu Qingxin group was given suspension of Zhuyu Qingxin capsules 0.054 g/(kg·d)by gavage,the risperidone group was given risperidone suspension 0.036 mg/(kg·d)by gavage,and the blank group and model group were given equal doses of distilled water by gavage,all once a day,continuously treated for 28 days.HE staining was used to observe the pathological morphology of rat brain tissue,LFB staining was used to observe the lesions in the white matter myelin sheath,and Western blot was used to observe the protein expression of inhibitor of differentiation 2(Id2),oligodendrocyte transcription factor 1(Olig1),and oligodendrocyte transcription factor 2(Olig2)in the frontal cortex,hippocampus,and cingulate tissue.Results In the model group,the nuclei of neurons in the brain tissue of rats showed significant pyknosis,with loose myelin tissue structure and obvious demyelinating lesions;Compared with the model group,the cell morphology was more regular,nuclear pyknosis was reduced,demyelinating lesions were improved,myelin tissue structure was more close in the Zhuyu Qingxin group and risperidone group,and the improvements in the Zhuyu Qingxin group were more significant.Compared with the blank group,the expression of Id2 protein in the frontal cortex,hippocampus,and cingulate tissue of the rats in the model group were significantly increased(P<0.05),while the expression of Olig1 and Olig2 proteins were significantly reduced(P<0.05);Compared with the model group,the expressi

关 键 词：精神分裂症 逐瘀清心胶囊 神经组织 髓鞘病变 分化抑制因子2 少突胶质细胞转录因子 

分 类 号：R-332[医药卫生]