Engineering Escherichia coli for high-yield production of ectoine  

作　　者：Daoan Wang Jiamin Chen Yang Wang Guocheng Du Zhen Kang 

机构地区：[1]The Science Center for Future Foods,Jiangnan University,Wuxi,214122,China [2]The Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,Jiangnan University,Wuxi,214122,China [3]The Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi,214122,China

出　　处：《Green Chemical Engineering》2023年第2期217-223,共7页绿色化学工程（英文）

基　　金：supported by the National Key Research and Development Program of China(2021YFC2100800);the Jiangsu Province Natural Science Fund for Distinguished Young Scholars(BK20200025);the National Natural Science Foundation of China(32000058);a grant from the Key Technologies R&D Program of Jiangsu Province(BE2019630);the National First-class Discipline Program of Light Industry Technology and Engineering(LITE2018-16).

摘　　要：Ectoine is a natural macromolecule protector and synthesized by some extremophiles.It provides protections against radiation-mediated oxidative damages and is widely used as a bioactive ingredient in pharmaceutics and cosmetics.To meet its growing commercial demands,we engineered Escherichia coli strains for the high-yield production of ectoine.The ectABC gene cluster from the native ectoine producer Halomonas elongata was intro-duced into different Escherichia coli(E.Coil)strains via plasmids and 0.8 g L^(-1)of ectoine was produced in flask cultures by engineered E.coli BL21(DE3).Subsequently,we designed the ribosome-binding sites of the gene cluster to fine-tune the expressions of genes ectA,ectB,and ectC,which increased the ectoine yield to 1.6 g L^(-1).After further combinatorial overexpression of Corynebacterium glutamicum aspartate kinase mutant(G1A,C932T)and the H.elongate aspartate-semialdehyde dehydrogenase to increase the supply of the precursor,the titer of ectoine reached to 5.5 g L^(-1)in flask cultures.Finally,the engineered strain produced 60.7 g L^(-1)ectoine in fed-batch cultures with a conversion rate of 0.25 g/g glucose.

关 键 词：ECTOINE Escherichia coli Metabolic engineering RBS design 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]