Characterization of glucose isomerase-producing bacteria and optimization of fermentation conditions for producing glucose isomerase using biomass  

作　　者：Aristide Laurel Mokale Kognou Chonlong Chio Janak Raj Khatiwada Sarita Shrestha Xuantong Chen Hongwei Li Yuen Zhu Zi-Hua Jiang Chunbao(Charles)Xu Wensheng Qin 

机构地区：[1]Department of Biology,Lakehead University,Thunder Bay,ON,P7B 5E1,Canada [2]Department of Chemical and Biochemical Engineering,Western University,London,ON,N6A 5B9,Canada [3]School of Environment and Resources,Shanxi University,Taiyuan,030006,China [4]Department of Chemistry,Lakehead University,Thunder Bay,ON,P7B 5E1,Canada

出　　处：《Green Chemical Engineering》2023年第2期239-249,共11页绿色化学工程（英文）

基　　金：supported by the Natural Science and Engineering Research Council of Canada(NSERC)Discovery Grant(RGPIN-2017-05366)to WQ.

摘　　要：Glucose isomerase(GI)is an enzyme with high potential applications.Characterization of GI producing bacteria with interesting properties from an industrial point of view is essential.Bacillus sp.,Paenarthrobacter sp.,Chryseobacterium sp.,Hymenobacter sp.,Mycobacterium sp.,and Stenotrophomonas sp.were isolated from soil samples.Optimization of enzyme production yield was investigated in various fermentation conditions using response surface methodology.All isolates exhibited maximum GI activity at 40℃,pH 6–8 after 4 days of incubation.A mixture of peptone/yeast extract or tryptone/peptone enhanced higher enzyme production.The same trend was observed in fermentation medium containing 1%xylose or 2%–2.5%wheat straw.This study advanced the knowledge of these bacterial isolates in promoting wheat straw as feedstock for the bio-based industry.

关 键 词：Cellulolytic bacteria Glucose/xylose isomerase 16S rRNA Biomass conversion 

分 类 号：TQ925.2[轻工技术与工程—发酵工程]