A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain  

作　　者：Yuting Shuai Yi Ju Yuanhang Li Dini Ma Lan Jiang Jingyu Zhang Gao-Yi Tan Xueting Liu Shenlin Wang Lixin Zhang Guang Liu 

机构地区：[1]State Key Laboratory of Bioreactor Engineering,and School of Biotechnology,East China University of Science and Technology(ECUST),Shanghai,200237,China [2]State Key Laboratory of Microbial Metabolism,Joint International Research Laboratory of Metabolic&Developmental Sciences,School of Life Sciences&Biotechnology,Shanghai Jiao Tong University,Shanghai,200240,China

出　　处：《Synthetic and Systems Biotechnology》2023年第2期213-219,共7页合成和系统生物技术（英文）

基　　金：supported by the National Natural Science Foundation of China(31900060);National Key Research and Development Program of China(2020YFA0907800,2022YFC3400200,2022YFA0912200);Natural Science Foundation of Shanghai(20ZR1414500);Shanghai Pilot Program for Basic Research-Shanghai Jiao Tong University(21TQ1400204).

摘　　要：Nucleic acid detection plays a key role in diverse diagnosis and disease control.Currently available nucleic acid detection techniques are challenged by trade-offs among speed,simplicity,precision and cost.Here,we described a novel method,designated SENSOR(Sulfur DNA mediated nucleic acid sensing platform),for rapid nucleic acid detection.SENSOR was developed from phosphorothioate(PT)-DNA and sulfur binding domain(SBD)which specifically binds double-stranded PT-modified DNA.SENSOR utilizes PT-DNA oligo and SBD as targeting module,which is linked with split luciferase reporter to generate luminescence signal within 10 min.We tested detection on synthesized nucleic acid and COVID-19 pseudovirus,achieving attomolar sensitivity combined with an amplification procedure.Single nucleotide polymorphisms(SNP)could also be discriminated.Indicating SENSOR a new promising nucleic acid detection technique.

关 键 词：PHOSPHOROTHIOATE Sulfur binding domain Nucleic acid detection 

分 类 号：Q63[生物学—生物物理学]