Transcriptomics reveals the effect of ammonia nitrogen concentration on Pseudomonas stutzeri F2 assimilation and the analysis of amtB function  

作　　者：Wei-lai Fu Pei-feng Duan Qiang Wang Ya-xin Liao Yun-shuang Wang Mei-juan Xu Hui-hui Jiang Xian Zhang Zhi-ming Rao 

机构地区：[1]The Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi,214122,PR China [2]The Key Laboratory of Functional Aquafeed and Culture Environment Control,Fujian DBN Huayou Aquatic Science and Technology Co.Ltd,Zhangzhou,363500,PR China [3]School of Biological and Environmental Engineering,Chaohu University,Hefei,238024,PR China

出　　处：《Synthetic and Systems Biotechnology》2023年第2期262-272,共11页合成和系统生物技术（英文）

基　　金：supported by Foundation of Fujian Key Laboratory of Functional Aquafeed and Culture Environment Control(No.FACE20200003);111 Project(111-2-06)and Jiangsu province“Collaborative Innovation Center for Advanced Industrial Fermentation”industry development program.

摘　　要：The biological treatment of wastewater with high concentrations of ammonia nitrogen has become a hot research issue,but there are limited reports on the mechanism of ammonia nitrogen utilization by microorganisms.In this paper,a transcriptomic approach was used to investigate the differences in gene expression at 500.0 mg/L(Amo 500)and 100.0 mg/L(Amo 100)ammonium concentrations to reveal the mechanism of ammonia nitrogen removal from water by Pseudomonas stutzeri F2.The transcriptome data showed 1015(459 up-regulated and 556 down-regulated)differentially expressed genes with functional gene annotation related to nitrogen source metabolism,glycolysis,tricarboxylic acid cycle,extracellular polysaccharide synthesis,energy conversion and transmembrane transport,revealing the metabolic process of ammonium nitrogen conversion to biological ni-trogen in P.stutzeri F2 through assimilation.To verify the effect of ammonium transporter protein(AmtB)of cell membrane on assimilation,a P.stutzeri F2-ΔamtB mutant strain was obtained by constructing a knockout plasmid(pK18mobsacB-ΔamtB),and it was found that the growth characteristics and ammonium removal rate of the mutant strain were significantly reduced at high ammonium concentration.The carbon source components and dissolved oxygen conditions were optimized after analyzing the transcriptome data,and the ammonium removal rate was increased from 41.23%to 94.92%with 500.0 mg/L ammonium concentration.The study of P.stutzeri F2 transcript level reveals the mechanism of ammonia nitrogen influence on microbial assimilation process and improvement strategy,which provides a new strategy for the treatment of ammonia nitrogen wastewater.

关 键 词：Ammonia nitrogen Pseudomonas stutzeri F2 TRANSCRIPTOMIC ASSIMILATION AMTB 

分 类 号：X70[环境科学与工程—环境工程]