Functionalization of in vivo tissue-engineered living biotubes enhance patency and endothelization without the requirement of systemic anticoagulant administration  

作　　者：Hongyu Yan Quhan Cheng Jianghua Si Songdi Wang Ye Wan Xin Kong Ting Wang Wenting Zheng Muhammad Rafique Xiaofeng Li Ju He Adam C.Midgley Yi Zhu Kai Wang 

机构地区：[1]Deling Kong a a Key Laboratory of Bioactive Materials for the Ministry of Education,College of Life Sciences,Nankai University,Tianjin,300071,China [2]Department of Ultrasound in Medicine,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,200233,China [3]Tianjin Key Laboratory of Urban Transport Emission Research,College of Environmental Science and Engineering,Nankai University,Tianjin,300071,China [4]State Key Laboratory of Experimental Hematology,National Clinical Research Center for Blood Diseases,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin,300020,China [5]Department of Vascular Surgery,Tianjin First Central Hospital,Nankai University,Tianjin,300192,China [6]Department of Physiology and Pathophysiology,Tianjin Medical University,Tianjin,300070,China

出　　处：《Bioactive Materials》2023年第8期292-305,共14页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(NSFC)projects 81921004(D.K.),82127808(D.K.),32222043(K.W.),82250610231(A.C.M.);National Key R&D Program of China 2022YFA1105102(K.W.);Tianjin Natural Science Foundation 20JCYBJC01150(K.W.);Tianjin Natural Science Foundation 18JCZDJC37600(K.W.);NCC Fund NCC2020PY18(K.W.);Tianjin"Project+Team"Key Training Foundation XC202035(K.W.).

摘　　要：Vascular regeneration and patency maintenance,without anticoagulant administration,represent key developmental trends to enhance small-diameter vascular grafts(SDVG)performance.In vivo engineered autologous biotubes have emerged as SDVG candidates with pro-regenerative properties.However,mechanical failure coupled with thrombus formation hinder translational prospects of biotubes as SDVGs.Previously fabricated poly(ε-caprolactone)skeleton-reinforced biotubes(PBs)circumvented mechanical issues and achieved vascular regeneration,but orally administered anticoagulants were required.Here,highly efficient and biocompatible functional modifications were introduced to living cells on PB lumens.The 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(DMPE)-PEG-conjugated anti-coagulant bivalirudin(DPB)and DMPE-PEG-conjugated endothelial progenitor cell(EPC)-binding TPS-peptide(DPT)modifications possessed functionality conducive to promoting vascular graft patency.Co-modification of DPB and DPT swiftly attained luminal saturation without influencing cell viability.DPB repellent of non-specific proteins,DPB inhibition of thrombus formation,and DPB protection against functional masking of DPT’s EPC-capture by blood components,which promoted patency and rapid endothelialization in rat and canine artery implantation models without anticoagulant administration.This strategy offers a safe,facile,and fast technical approach to convey additional functionalization to living cells within tissue-engineered constructs.

关 键 词：In vivo tissue-engineering Biotube Living tissue modification ANTICOAGULATION Rapid endothelialization 

分 类 号：R318[医药卫生—生物医学工程]