Microenvironment responsive nanocomposite hydrogel with NIR photothermal therapy,vascularization and anti-inflammation for diabetic infected wound healing  

作　　者：Shuangli Zhu Bangjiao Zhao Maocai Li Hao Wang Jiayi Zhu Qingtao Li Huichang Gao Qi Feng Xiaodong Cao 

机构地区：[1]School of Materials Science and Engineering,South China University of Technology,Guangzhou,510641,PR China [2]National Engineering Research Centre for Tissue Restoration and Reconstruction,South China University of Technology,Guangzhou,510006,PR China [3]School of Medicine,South China University of Technology,Guangzhou,510006,PR China [4]Key Laboratory of Biomedical Engineering of Guangdong Province,South China University of Technology,Guangzhou,510006,PR China [5]Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education,South China University of Technology,Guangzhou,510006,PR China [6]Zhongshan Institute of Modern Industrial Technology of SCUT,Zhongshan,Guangdong,528437,PR China

出　　处：《Bioactive Materials》2023年第8期306-320,共15页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(Grant No.52272276,52073103,52203164);the Key-Area Research and Development Program of Guangdong Province(Grant No.2020B090924004);the funds for Zhongshan Innovation Project of high-end Scientific Research Institutions(Grant No.2020AG020);the Fundamental Research Funds for the Central Universities(No.2022ZYGXZR105);Guangdong Basic and Applied Basic Research Foundation(Grant No.2021A1515010905).

摘　　要：Bacterial infection,excessive inflammation and damaging blood vessels network are the major factors to delay the healing of diabetic ulcer.At present,most of wound repair materials are passive and can’t response to the wound microenvironment,resulting in a low utilization of bioactive substances and hence a poor therapeutic effect.Therefore,it’s essential to design an intelligent wound dressing responsive to the wound microenvironment to achieve the release of drugs on-demand on the basis of multifunctionality.In this work,metformin-laden CuPDA NPs composite hydrogel(Met@CuPDA NPs/HG)was fabricated by dynamic phenylborate bonding of gelatin modified by dopamine(Gel-DA),Cu-loaded polydopamine nanoparticles(CuPDA NPs)with hyaluronic acid modified by phenyl boronate acid(HA-PBA),which possessed good injectability,self-healing,adhesive and DPPH scavenging performance.The slow release of metformin was achieved by the interaction with CuPDA NPs,boric groups(B-N coordination)and the constraint of hydrogel network.Metformin had a pH and glucose responsive release behavior to treat different wound microenvironment intelligently.Moreover,CuPDA NPs endowed the hydrogel excellent photothermal responsiveness to kill bacteria of>95%within 10 min and also the slow release of Cu^(2+)to protect wound from infection for a long time.Met@CuPDA NPs/HG also recruited cells to a certain direction and promoted vascularization by releasing Cu^(2+).More importantly,Met@CuPDA NPs/HG effectively decreased the inflammation by eliminating ROS and inhibiting the activation of NF-κB pathway.Animal experiments demonstrated that Met@CuPDA NPs/HG significantly promoted wound healing of diabetic SD rats by killing bacteria,inhibiting inflammation,improving angiogenesis and accelerating the deposition of ECM and collagen.Therefore,Met@CuPDA NPs/HG had a great application potential for diabetic wound healing.

关 键 词：Diabetic ulcer Hydrogel dressing Cu-loaded polydopamine nanoparticles METFORMIN Photothermal responsiveness Angiogenesis and anti-inflammation 

分 类 号：TQ427.26[化学工程] R318[医药卫生—生物医学工程] R587.1[医药卫生—基础医学]