Generation and metabolomic characterization of functional ductal organoids with biliary tree networks in decellularized liver scaffolds  

作　　者：Jiaxian Chen Shiwen Ma Hui Yang Xi Liang Heng Yao Beibei Guo Deying Chen Jing Jiang Dongyan Shi Jiaojiao Xin Keke Ren Xingping Zhou Yun Li Lei Geng Jun Li 

机构地区：[1]State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,National Clinical Research Center for Infectious Diseases,National Medical Center for Infectious Diseases,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,The First Affiliated Hospital,Zhejiang University School of Medicine,79 Qingchun Rd.,Hangzhou,310003,China [2]Precision Medicine Center,Taizhou Central Hospital(Taizhou University Hospital),Taizhou,318000,China [3]Institute of Pharmaceutical Biotechnology,Zhejiang University School of Medicine,Hangzhou,310013,China [4]Division of Hepatobiliary and Pancreatic Surgery,Department of Surgery,The First Affiliated Hospital,Zhejiang University School of Medicine,79 Qingchun Rd.,Hangzhou,310003,China

出　　处：《Bioactive Materials》2023年第8期452-464,共13页生物活性材料（英文）

基　　金：supported by the National Key R&D Program of China(2022YFA1104102,2022YFA1104603,2022YFC2304801);the National Natural Science Foundation of China(81830073,82272426);the National Provincial special support program for high-level personnel recruitment(Ten-thousand Talents Program,2018RA4016);Zhejiang Public Welfare Project(LY21H030007,LGF21H200006).

摘　　要：Developing functional ductal organoids(FDOs)is essential for liver regenerative medicine.We aimed to construct FDOs with biliary tree networks in rat decellularized liver scaffolds(DLSs)with primary cholangiocytes isolated from mouse bile ducts.The developed FDOs were dynamically characterized by functional assays and metabolomics for bioprocess clarification.FDOs were reconstructed in DLSs retaining native structure and bioactive factors with mouse primary cholangiocytes expressing enriched biomarkers.Morphological assessment showed that biliary tree-like structures gradually formed from day 3 to day 14.The cholangiocytes in FDOs maintained high viability and expressed 11 specific biomarkers.Basal-apical polarity was observed at day 14 with immunostaining for E-cadherin and acetylatedα-tubulin.The rhodamine 123 transport assay and active collection of cholyl-lysyl-fluorescein exhibited the specific functions of bile secretion and transportation at day 14 compared to those in monolayer and hydrogel culture systems.The metabolomics analysis with 1075 peak pairs showed that serotonin,as a key molecule of the tryptophan metabolism pathway linked to biliary tree reconstruction,was specifically expressed in FDOs during the whole period of culture.Such FDOs with biliary tree networks and serotonin expression may be applied for disease modeling and drug screening,which paves the way for future clinical therapeutic applications.

关 键 词：CHOLANGIOCYTE Decellularized scaffold Ductal organoid Extracellular matrix 

分 类 号：R318[医药卫生—生物医学工程]