Bioactive superparamagnetic iron oxide-gold nanoparticles regulated by a dynamic magnetic field induce neuronal Ca^(2+)influx and differentiation  

作　　者：Elias Georgas Muzhaozi Yuan Jingfan Chen Ya Wang Yi-Xian Qin 

机构地区：[1]Department of Biomedical Engineering,The State University of New York at Stony Brook,Stony Brook,NY,United States [2]J.Mike Walker’66 Department of Mechanical Engineering,Texas A&M University,College Station,TX,United States [3]Department of Biomedical Engineering,Texas A&M University,College Station,TX,United States [4]Department of Electrical and Computer Engineering,Texas A&M University,College Station,TX,United States

出　　处：《Bioactive Materials》2023年第8期478-489,共12页生物活性材料（英文）

基　　金：funded by the United States National Science Foundation(NSF)(CAREER Award#CMMI 1851635,Y.W.and GCR awards#ECCS 2021081(Y.W.);#ECCS 2020867(Y-X.Q).

摘　　要：Treating neurodegenerative diseases,e.g.,Alzheimer’s Disease,remains a significant challenge due to the limited neuroregeneration rate in the brain.The objective of this study is to evaluate the hypothesis that external magnetic field(MF)stimulation of nerve growth factor functionalized superparamagnetic iron oxide-gold(NGF-SPIO-Au)nanoparticles(NPs)can induce Ca^(2+)influx,membrane depolarization,and enhance neuron differentiation with dynamic MF(DMF)outperforming static MF(SMF)regulation.We showed the that total intracellular Ca^(2+)influx of PC-12 cells was improved by 300%and 535%by the stimulation of DMF(1 Hz,0.5 T,30min)with NGF-SPIO-Au NPs compared to DMF alone and SMF with NGF-SPIO-Au NPs,respectively,which was attributed to successive membrane depolarization.Cellular uptake performed with the application of sodium azide proved that DMF enhanced cellular uptake of NGF-SPIO-Au NPs via endocytosis.In addition,DMF upregulated both the neural differentiation marker(β3-tubulin)and the cell adhesive molecule(integrin-β1)with the existence of NGF-SPIO-Au NPs,while SMF did not show these effects.The results imply that noninvasive DMF-stimulated NPs can regulate intracellular Ca^(2+)influx and enhance neuron differentiation and neuroregeneration rate.

关 键 词：Calcium influx Membrane potential Dynamic magnetic fields NEUROREGENERATION SPIO-Au Core-shell nanoparticles 

分 类 号：TB383.1[一般工业技术—材料科学与工程] R318[医药卫生—生物医学工程]