基于金纳米粒子修饰的喜树碱纳米药物的构建及其抗癌活性  

Construction and anti-cancer activity of gold nanoparticles decorated camptothecin nanocrystals

作　　者：宋文静 刘涛[2] 王际辉[1,3] 詹宏磊 SONG Wenjing;LIU Tao;WANG Jihui;ZHAN Honglei(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China;School of Environment,Dalian University of Technology,Dalian 116024,China;School of Chemical Engineering and Energy Technology,Dongguan University of Technology,Dongguan 523808,China)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]大连理工大学环境学院,辽宁大连116024 [3]东莞理工学院化学工程与能源技术学院,广东东莞523808

出　　处：《大连工业大学学报》2023年第3期169-175,共7页Journal of Dalian Polytechnic University

基　　金：辽宁省教育厅科学研究经费项目(LJKZ0539).

摘　　要：金纳米粒子具有抗癌及高光热转换效率的潜能,以透明质酸为稳定剂、金纳米粒子为还原及固定层,将化疗与光热疗法两种模式进行组合,合成了金纳米粒子修饰的纳米药物晶体(CPT-HA-AuNPs)。利用DLS、SEM等技术对CPT-HA-AuNPs的理化性质及药物控释行为进行表征,利用MTT、细胞摄取实验及AnnexinV-FITC/PI双染法对其生物活性进行了研究。结果表明,CPT-HA-AuNPs呈杆状纳米晶体结构,粒径(238.60±4.51)nm,Zeta电位(-47.29±0.84)mV,显示出较好的稳定性;具有较高的光热转换效率,并展现出光热及pH双响应性释放行为。与对照组相比,CPT-HA-AuNPs在NIR光激发条件下表现出显著提高的MDA-MB-231细胞摄取率、体外抗癌活性及促凋亡作用。According to the potential of gold nanoparticles for anti-cancer and high photo thermal conversion efficiency,gold nanoparticles modified drug nanocrystals(CPT-HA-AuNPs)were synthesized with hyaluronic acid as the stabilizer and gold nanoparticles as the reduction and fixation layer.Chemotherapy and photothermal therapy are combined to improve the overall anti-cancer efficiency of drugs.The physicochemical properties and drug controlled release behavior of CPT-HA-AuNPs were characterized by DLS and SEM.Their biological activity was studied by MTT,cell uptake experiments and Annexin V-FITC/PI double staining method.The results showed that CPT-HA-AuNPs exhibited a rod-shaped nanocrystalline structure with a particle size of(238.60±4.51)nm and Zeta potential of(-47.29±0.84)mV with good stability.CPT-HA-AuNPs have high photothermal conversion efficiency and dual responsive release behavior of photothermal and pH.Compared with the control group,CPT-HA-AuNPs significantly increased MDA-MB-231 cell uptake in vitro anticancer activity,and proapoptotic effects by NIR stimulation.

关 键 词：喜树碱 金纳米粒子 纳米药物晶体 乳腺癌 光热疗法 

分 类 号：R944.2[医药卫生—药剂学]