环状RNA hsa_circ_0001479在胃癌中的表达及功能研究  

Expression and function of hsa_circ_0001479 in gastric cancer

作　　者：施欣 景蓉蓉[1] 王洁[1,2] 刘思楠 崔明 SHI Xin;JING Rongrong;WANG Jie;LIU Sinan;CUI Ming(Department of Laboratory Medicine,the Affiliated Hospital of Nantong University,Nantong 226001;Medical School of Nantong University,Nantong 226001,China)

机构地区：[1]南通大学附属医院检验科,江苏南通226001 [2]南通大学医学院,江苏南通226001

出　　处：《南京医科大学学报（自然科学版）》2023年第5期617-625,共9页Journal of Nanjing Medical University(Natural Sciences)

基　　金：中国博士后科学基金资助项目(2020M681688)。

摘　　要：目的:探讨hsa_circ_0001479在胃癌(gastric cancer,GC)组织中的表达情况及其对胃癌细胞生物学行为的影响。方法:建立实时荧光定量PCR(RT-qPCR)检测hsa_circ_0001479表达量的方法并进行方法学性能评价;通过RT-qPCR检测76对胃癌组织和配对癌旁组织中hsa_circ_0001479的表达水平并分析其表达量与临床病理参数的关系;构建针对hsa_circ_0001479的小干扰RNA(small interfering RNA,siRNA)和过表达载体,转染至胃癌细胞株,通过CCK-8实验、克隆形成实验检测细胞增殖能力,流式细胞术检测细胞周期分布,划痕实验检测细胞横向迁移能力,Transwell实验检测细胞侵袭和迁移能力。结果:RT-qPCR检测hsa_circ_0001479表达量的方法具有良好的精密度、正确度和线性;hsa_circ_0001479在胃癌组织中表达增高且表达水平与肿瘤大小、浸润深度、淋巴结转移、TNM分期和CA19-9水平有关;细胞功能实验显示,在胃癌细胞株中干扰hsa_circ_0001479的表达可抑制胃癌细胞的增殖、侵袭和迁移,过表达hsa_circ_0001479则呈现相反的结果。结论:胃癌组织中高表达的hsa_circ_0001479具有促进胃癌细胞增殖、侵袭和迁移的功能。Objective:The current study aims to explore the expression of hsa_circ_0001479 in gastric cancer(GC)tissues and its effect on biological behavior of GC cells.Methods:The method for detecting the expression of hsa_circ_0001479 with real⁃time fluorescence quantitative polymerase chain reaction(RT⁃qPCR)was established and evaluated.Expression level of hsa_circ_0001479 in 76 pairs of GC and para⁃cancerous tissues was measured by RT⁃qPCR and relationship between the expression level and clinicopathological factors was analyzed.Small interfering RNA(siRNA)and overexpression vector of hsa_circ_0001479 were constructed and transfected into GC cell lines.Cell proliferation was detected by CCK⁃8 assay and clone formation assay.Cell cycle distribution was detected by flow cytometry.Cell migration was detected by wound healing assay and Transwell assay.Cell invasion was detected by transwell assay.Results:Using RT⁃qPCR to detect the expression of hsa_circ_0001479 possessed good precision,accuracy and linear range.Hsa_circ_0001479 was upregulated in GC tissues and the expression level was related to tumor size,depth of invasion,lymph node metastasis,TNM stage and CA19⁃9.Silencing of hsa_circ_0001479 inhibited the proliferation,invasion and migration of GC cells while overexpression of hsa_circ_0001479 showed the opposite results.Conclusion:The carrent study found that hsa_circ_0001479 was upregulated in GC tissues and could promote GC cell proliferation,invasion and migration.

关 键 词：胃癌 环状RNA hsa_circ_0001479 分子功能 

分 类 号：R735.2[医药卫生—肿瘤]