lncRNA DBH-AS1通过靶向miR-1291影响婴幼儿血管瘤内皮细胞增殖和凋亡  

lncRNA DBH-AS1 Affects the Proliferation and Apoptosis of Infantile Hemangioma Endothelial Cells by Targeting miR-1291

作　　者：覃佳佳 郭莹 吴俊 刘洋 QIN Jiajia;GUO Ying;WU Jun;LIU Yang(Department of Pediatrics,Wuhan Asia General Hospital,Wuhan 430056,China)

机构地区：[1]武汉亚心总医院儿科,武汉430056

出　　处：《中国细胞生物学学报》2023年第4期578-587,共10页Chinese Journal of Cell Biology

基　　金：武汉市卫生局科研项目(批准号:WG16D16)资助的课题。

摘　　要：该文旨在探讨长链非编码RNA(lncRNA)DBH-AS1对婴幼儿血管瘤内皮细胞(HemECs)增殖和凋亡的影响及可能机制。收集49个婴幼儿的血管瘤组织及瘤旁正常皮下组织,利用qRT-PCR检测血管瘤组织及瘤旁正常皮下组织中DBH-AS1和miR-1291的表达情况,通过Pearson相关性分析评价婴幼儿血管瘤患者血管瘤组织中DBH-AS1和miR-1291表达水平的相关性。CCK-8法和克隆形成实验检测细胞增殖情况,流式细胞术检测细胞凋亡情况,Westernblot检测增殖、凋亡相关蛋白的表达情况。血管瘤组织中DBH-AS1的表达水平高于瘤旁正常皮下组织(P<0.05),而miR-1291表达水平低于瘤旁正常皮下组织(P<0.05),Pearson相关性分析结果显示,血管瘤组织中DBH-AS1表达水平与miR-1291表达水平呈负相关(r=–0.887,P<0.01)。与si-NC组或miR-NC组比较,si-DBH-AS1组和miR-1291组细胞增殖活性和Ki-67、PCNA蛋白表达水平降低(P<0.05),细胞凋亡率和cleaved-caspase9、cleaved-caspase3蛋白表达水平升高(P<0.05)。DBHAS1可靶向结合miR-1291,且si-DBH-AS1组HemECs中miR-1291表达水平高于si-NC组(P<0.05)。下调miR-1291逆转干扰DBH-AS1对HemECs增殖和凋亡的作用(P<0.05)。干扰DBH-AS1表达可阻碍HemECs增殖,并促进细胞凋亡,其可能通过负调控miR-1291发挥作用。This study aims to investigate the effect of lncRNA(long non-coding RNA)DBH-AS1 on the proliferation and apoptosis of infantile HemECs(hemangioma endothelial cells)and its possible mechanism.Hemangioma tissue and normal subcutaneous tissue adjacent to tumor in 49 infants were collected.qR T-PCR was used to detect the expression of DBH-AS1 and miR-1291 in hemangioma tissue and adjacent normal subcutaneous tissue,and Pearson correlation analysis was used to evaluate the correlation between the expression levels of DBH-AS1 and miR-1291 in hemangioma tissues of infant hemangioma patients.Cell proliferation was detected by CCK-8 assay and clonal formation assay.Cell apoptosis was detected by flow cytometry.The expressions of proliferation and apoptosis related proteins were detected by Western blot.Dual-luciferase reporter assay was used to verify the regulatory relationship between DBH-AS1 and miR-1291.The expression of DBH-AS1 in hemangioma tissue was higher than that in adjacent normal subcutaneous tissue(P<0.05),while the expression of miR-1291 was lower than that in adjacent normal subcutaneous tissue(P<0.05).Pearson correlation analysis showed that the expression level of DBH-AS1 in hemangioma tissue was negatively correlated with that of miR-1291(r=-0.887,P<0.01).Compared with the si-NC group or miR-NC group,the proliferation activity of HemECs and the protein expression levels of Ki-67 and PCNA in the si-DBH-AS1 group and miR-1291 group were reduced(P<0.05),but the apoptosis rate and the protein expression levels of cleaved-caspase9 and cleaved-caspase3 were increased(P<0.05).DBH-AS1 could target miR-1291,and the expression level of miR-1291 in HemECs in the si-DBH-AS1 group was higher than that in the si-NC group(P<0.05).Downregulation of miR-1291 reversed the effect of interfering DBH-AS1 on the proliferation and apoptosis of HemECs(P<0.05).Interference with DBH-AS1 expression inhibits HemECs proliferation and promotes apoptosis,possibly through the negative regulation of miR-1291.

关 键 词：血管瘤 DBH-AS1 miR-1291 细胞增殖 凋亡 

分 类 号：R732.2[医药卫生—肿瘤]