HEMA对人乳牙牙髓干细胞分化潜能的影响  

Effects of HEMA on the in vitro odontoblastic differentiation potential of human pulp stem cells derived from deciduous teeth

作　　者：孙琴 吕迪 陈美玲 SUN Qin;LV Di;CHEN Mei-ling(Department of Stomatology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院口腔医学中心,湖北武汉430030

出　　处：《临床口腔医学杂志》2023年第5期279-282,共4页Journal of Clinical Stomatology

基　　金：华中科技大学教学研究基金项目(2021035);国家自然科学基金项目(82001093);湖北省卫健委青年人才项目(WJ2021Q026)。

摘　　要：目的:探究HEMA对人乳牙牙髓干细胞向成牙本质细胞分化的影响。方法:将不同浓度的HEMA加入到SHED细胞中分别培养24 h、48 h和72 h后,MTT实验检测其毒性作用。将50 mg/mL抗坏血酸,10 mmol/Lβ-甘油磷酸钠和100 nmol/L地塞米松加入含10%胎牛血清的DMEM中制备矿化诱导液,无毒浓度HEMA(0.1 mmol/L和0.25 mmol/L)下,诱导SHED向成牙本质细胞分化,素红染色观察矿化结节的形成。结果:当HEMA浓度超过0.5 mmol/L,培养时间超过48 h时,HEMA对SHED细胞有毒性作用。长时间暴露于无毒浓度HEMA中,SHED细胞的分化潜能受到抑制。结论:HEMA可抑制SHED细胞向成牙本质细胞分化。Objective:To investigate the effects of HEMA on the odontoblastic differentiation potential of dental pulp stem cells derived from deciduous teeth.Methods:Different concentrations of HEMA were added into SHED cells and then cultured for 24 h,48 h and 72 h,respectively.Cytotoxicity was evaluated with the MTT assay.For preparing mineralization induction solution,50 mg/mL ascorbic acid,10 mmol/L sodiumβ-glycerophosphate and 100 nmol/L dexamethasone were added to DMEM containing 10%fetal bovine serum.SHED cells were induced into odontoblast differentiation at nontoxic concentrations of SHED(0.1 mmol/L and 0.25 mmol/L).Alizarin red s staining was performed to detect changes in production of the extracellular matrix mineralization.Results:When the concentration of HEMA overdoes 0.5 mmol/L and the incubation time exceeds 48 h,HEMA had a toxic effect on SHED cells.Long-term exposure to nontoxic concentrations of HEMA significantly inhibited the differentiation of SHED.Conclusion:HEMA can severely inhibit the odontoblastic differentiation potential of human pulp stem cells derived from deciduous teeth.

关 键 词：甲基丙烯酸羟乙酯 乳牙牙髓干细胞 细胞分化 

分 类 号：Q813[生物学—生物工程] R78[医药卫生—口腔医学]