碳源诱导下D.desulfuricans sub sp.EPS特性及对Cd(Ⅱ)的吸附  

The EPS characteristics of D.desulfuricans sub sp.and its adsorption performance for Cd(Ⅱ)under carbon source induction

作　　者：黄祥武 宋卫锋[1] 杨佐毅[1] 戴文灿[1] 甘雨 连泽阳 周文斌 陈丽瑶 吴芷昕 HUANG Xiang-wu;SONG Wei-feng;YANG Zuo-yi;DAI Wen-can;GAN Yu;LIAN Ze-yang;ZHOU Wen-bin;CHENG Li-yao;WU Zhi-xin(School of Environmental Science and Engineering,Guangdong University of Technology,Guangzhou 510006,China)

机构地区：[1]广东工业大学环境科学与工程学院,广东广州510006

出　　处：《中国环境科学》2023年第6期2889-2898,共10页China Environmental Science

基　　金：国家自然科学基金资助项目(42277220)。

摘　　要：通过不同浓度下3种碳源(HCOONa、CH_(3)COONa和C_(6)H_(12)O_(6))对Desulfovibrio desulfuricans sub sp.(D.desulfuricans sp.)进行胁迫/诱导培养,研究了胁迫/诱导前后EPS组分变化以及对Cd(Ⅱ)吸附性能的影响.结果表明,当碳源HCOONa的胁迫浓度为3.0g/L时,胁迫/诱导效果最明显,EPS产量达到1709.78mg/g VSS,其中蛋白质含量最高,达到1516.68mg/g VSS,较胁迫/诱导前提高了244.93%.在此条件下,HCOONa-EPS对于Cd(Ⅱ)的吸附量最高,为1081.95mg/g EPS,提高了99.47%,且吸附过程符合二级动力学规律.三维荧光(3D-EEM)测试表明,胁迫/诱导后EPS中蛋白质含量尤其类色氨酸含量大幅增加;傅里叶红外光谱(FTIR)测试表明,胁迫后C=O、N-H/C-N、C-O-C等官能团均大量增加,在吸附Cd(Ⅱ)中发挥了重要作用;X光电子能谱(XPS)结果表明,HCOONa胁迫/诱导后,EPS中C-O/C-N、C=O、C=N等官能团的浓度明显增加,可能是吸附Cd(Ⅱ)的主要基团.Through three kinds of carbon source(HCOONa,CH_(3)COONa and C_(6)H_(12)O_(6)),under different concentrations of Desulfovibrio desulfuricans sub sp.(D.desulfuricans sp.)for stress/induction training,the changes in EPS fractions before and after stress/induction and changes in the sorption properties of Cd(Ⅱ)were investigated.The results showed that when the stress concentration of HCOONa was 3.0g/L,the stress/induction effect was the most obvious,EPS production reached 1709.78mg/g VSS,and the protein yield in EPS was the highest,reaching 1516.68mg/g VSS,which was 244.93%higher than that before stress/induction.Under these conditions,HCOONa-EPS showed the highest adsorption of Cd(Ⅱ)at 1081.95mg/g EPS,an increase of 99.47%,and the adsorption process conforms to the secondary kinetic law.Three-dimensional fluorescence(3D-EEM)tests showed that the protein content,especially tryptophan-like content,increased significantly in EPS after stress/induction.Fourier infrared spectroscopy(FTIR)test showed that C=O,N-H/C-N,C-O-C and other functional groups increased greatly after stress,which played an important role in adsorption of Cd(Ⅱ).X-ray electron spectroscopy(XPS)showed that after the stress/induction of carbon source HCOONa,the concentration of functional groups such as C-O/C-N,C=O,C=N and other functional groups in EPS increased significantly,which may be the main group for adsorption of Cd(Ⅱ).

关 键 词：EPS 蛋白质 碳源 Cd(Ⅱ) 

分 类 号：X172[环境科学与工程—环境科学]