鄱阳湖水系细菌群落结构特征及构建机制  

Structure and assembly processes of bacterial communities in Poyang Lake basin

作　　者：折远洋 王鹏[1] 丁明军[1] 聂明华[1] 黄高翔[1] 张华 黄益平[1] 曹英杰 SHE Yuan-yang;WANG Peng;DING Ming-jun;NIE Ming-hua;HUANG Gao-xiang;ZHANG Hua;HUANG Yi-ping;CAO Ying-jie(Key Laboratory of Poyang Lake Wetland and Watershed Research,Ministry of Education,School of Geography and Environment,Jiangxi Normal University,Nanchang 330022,China;School of History Culture and Tourism,Longnan Teachers College,Longnan 742500,China;School of Environmental Science and Engineering,Sun Yat-sen University,Guangzhou 510006,China)

机构地区：[1]江西师范大学地理与环境学院,鄱阳湖湿地与流域研究教育部重点实验室,江西南昌330022 [2]陇南师范高等专科学校历史文化与旅游学院,甘肃陇南742500 [3]中山大学环境科学与工程学院,广东广州510006

出　　处：《中国环境科学》2023年第6期3129-3140,共12页China Environmental Science

基　　金：国家自然科学基金资助项目(42167013);鄱阳湖湿地与流域研究教育部重点实验室(江西师范大学)开放基金资助项目(PK2019002)。

摘　　要：为探讨不同时空尺度下河流细菌群落结构和构建机制,于枯水期(10~翌年1月)和丰水期(4~8月)在鄱阳湖水系(五河七口)共进行8次水样采集,基于高通量测序技术分析了细菌群落结构、网络交互作用及构建机制.结果表明:Shannon指数丰水期(5.12)显著高于枯水期(4.45),Chao1指数不同水期间差异不显著,丰水期与枯水期内α多样性均存在显著差异.不同站点间细菌群落α多样性差异不显著,β多样性存在差异.假单胞菌门(Pseudomonadota,34.72%)、放线菌门(Actinobacteriota,32.35%)、蓝藻菌门(Cyanobacteria,13.21%)和拟杆菌门(Bacteroidota,10.81%)是鄱阳湖水系的优势菌门,相对于枯水期,丰水期Pseudomonadota和Actinobacteriota相对丰度显著下降,Cyanobacteria和Bacteroidota显著上升.水温(WT)和平均河网长度(MDSL)是影响门水平细菌群落结构的主要环境因子.水体细菌群落的生态网络相互协作、竞争关系并存,丰水期物种间复杂性和稳定性强于枯水期.流量(WF)、WT和溶解氧(DO)与共生网络显著相关.确定性过程主导细菌群落构建机制,枯水期确定性过程贡献强于丰水期,不同时间尺度下共生网络模式和构建机制存在一定差异.To investigate the structure and assembly processes of bacterial communities across different spatial-temporal scales,a total of eight surface water samples were collected in Poyang Lake basin(from seven hydrological stations)during the dry season(October to January of the following year)and wet season(April to August),and high-throughput sequencing technology was utilized to examine the bacterial community's structure,assembly processes,and co-occurrence networks.The results demonstrated that the Shannon index was significantly higher(5.12)during the wet season compared to the dry season(4.45),while the Chao1index did not differ significantly between the seasons.Moreover,significant differences inαdiversity were observed within the same season,while no significant differences were detected in theαandβdiversity of bacterial communities in space.The dominant phyla in the Poyang Lake basin were Pseudomonadota(34.72%),Actinobacteria(32.35%),Cyanobacteria(13.21%),and Bacteroidetes(10.81%).The relative abundance of Pseudomonadota and Actinobacteriota decreased significantly,while Cyanobacteria and Bacteroidota significantly increased during the wet season compared to the dry season.Water temperature(WT)and mean stream network length(MDSL)were identified as the primary environmental factors influencing the structure of the bacterial community at the phyla-level.The co-occurrence networks of bacterial communities in water bodies interacted with each other in collaborative and competitive relationships,which were stronger,more complex,and stable during the wet season than in the dry season.Additionally,the complexity and stability of the co-network were significantly influenced by both WT and dissolved oxygen(DO).The assembly of bacterial communities was predominantly dominated by deterministic processes,with a stronger contribution from these processes during the dry season than in the wet season.Finally,some differences in co-network patterns and assembly processes were observed across different time scales.

关 键 词：鄱阳湖水系 细菌群落 共生网络 构建机制 尺度效应 

分 类 号：X172[环境科学与工程—环境科学]