球形棕囊藻溶藻菌的分离鉴定及溶藻作用研究  

Isolation,Identification of Algicidal Bacteria Against Phaeocystis globosa and Its Algicidal Effect

作　　者：强俊磊 陈向东[1] 汪辉[1] 田宇航 郑欣悦 QIANG Junlei;CHEN Xiangdong;WANG Hui;TIAN Yuhang;ZHEN Xinyue(School of Life Sciences and Technology,China Pharmaceutical University,Nanjing Jiangsu 211198)

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京211198

出　　处：《现代农业科技》2023年第11期150-155,共6页Modern Agricultural Science and Technology

摘　　要：为了从广西北部湾海域分离筛选和鉴定针对球形棕囊藻的溶藻细菌,研究其溶藻特性。本研究采用梯度稀释和平板划线法从海水水样中分离纯化溶藻菌,菌藻共培养后,通过观察颜色和测定叶绿素a含量筛选溶藻菌,形态观察和细菌16S rDNA测序鉴定溶藻菌;通过光学显微镜观察溶藻菌的溶藻过程,测定溶藻菌菌液、菌体重悬液、无菌滤液的溶藻率,分析其主要溶藻方式;通过测定菌藻共培养后藻液的叶绿素a含量,研究溶藻菌的生长时期、菌液添加量、球形棕囊藻的生长阶段、光照等对溶藻效果的影响。结果表明,此次试验分离筛选出一株具有高效溶藻作用的溶藻菌PG47,鉴定为高地芽孢杆菌。PG47间接抑藻,溶藻活性物质可以破坏藻细胞的细胞壁和细胞膜。对数期以后的菌液均具有较好的溶藻活性,且当菌液与藻液体积比达到5%以后,溶藻率高达80%,对处于迟缓期、对数期、平台期的球形棕囊藻均具有高效的溶藻效果;在不同光照条件下,溶藻率均维持在60%以上,适用性广。综上,本研究筛选出的高地芽孢杆菌PG47溶藻活性较高,且具有控制球形棕囊藻赤潮的潜力。In order to isolate,screen and identify algicidal bacteria against Phaeocystis globosa from Beibu Gulf of Guangxi,and study its algicidal activity,this study isolated and purified algicidal bacteria by gradient dilution and plate streaking method.Algicidal bacteria were screened by observing color and determining chlorophyll a content after coculture of bacteria and algae.Algicidal bacteria were identified by morphological observation and 16S rDNA sequences analysis.The algicidal progress was observed with light microscope.The main algicidal way were analyzed by measuring the algicidal rate of bacterial culture,washed bacteria and sterile filtrate.The algicidal effects of co-culture time of bacteria and algae,inoculum additive amount,growth stage of Phaeocystis globosa and light conditions were studied by measuring the content of chlorophyll a after co-culture of bacteria and algae.The results showed that an algicidal bacterium(PG47)with high-efficiency algicidal activity was isolated and identified as Bacillus altitudinis in this experiment.The algicidal mode of the strain PG47 was indirect,and its extracellular secretions could destroy the cell wall and cell membrane.The bacterial suspension after log phase all showed significant algicidal activity.The algicidal rate achieved 80%when the volume ratio of bacterial to algal suspension was more than 5%.It has highly algicidal effect againt Phaeocystis globosa in lag phase,log phase and stationary phase,and kept above 60%under different light conditions.In conclusion,the isolated Bacillus altitudinis PG47 has high algicidal activity,showing potential for the control of Phaeocystis globosa red tide.

关 键 词：球形棕囊藻 溶藻细菌 分离 鉴定 溶藻特性 

分 类 号：X172[环境科学与工程—环境科学] X55