miR320a靶向FOXQ1调控肝癌细胞增殖和侵袭的实验研究  

Experimental study of miR-320a targeting FOXQ1 to regulate the proliferation and invasion of hepatoellular carcinoma cells

作　　者：马春阳 周益龙[1] 陈石 邵冰峰[1] MA Chun-yang;ZHOU Yi-long;CHEN Shi(Department of Hepalobiliary Surgery,Cancer Hospital Affiliated to Nantong University,Nantong,Jiangsu 226000)

机构地区：[1]南通大学附属肿瘤医院肝胆外科,南通226000

出　　处：《肝胆外科杂志》2023年第2期147-153,共7页Journal of Hepatobiliary Surgery

基　　金：2019年高层次卫生人才“六个一工程”拔尖人才科研项目(LGY201960);2020年度南通市市级科技计划(指导性)项目(JCZ20132)。

摘　　要：目的探讨miR-320a对肝癌细胞增殖和侵袭的影响及分子机制。方法采用荧光定量PCR检测不同肝癌细胞系HepG2、SMMC-7721、Bel-7402.QGY-7701、QGY-7703和正常肝细胞中HL-7702中miR-320a的表达水平;细胞克隆形成实验和Transwell小室法检测miR320a对肝癌细胞增殖、迁移和侵袭的影响;Westernblot检测细胞上皮-间质转化相关的E-.cadherin、N-cadherin和vimentin的表达水平;双荧光素酶报告基因实验、qPCR和W estern blot验证miR-320a对叉头框蛋白Q1的靶向作用。采用裸鼠皮下移植瘤实验研究miR-320a通过靶向FOXQ1对HepG2细胞体内成瘤的影响。结果miR-320a在肝癌细胞中表达下调,过表达miR-320a抑制肝癌细胞增殖、迁移和侵袭,抑制EMT。MiR-320a通过靶向抑制FOXQ1抑制HepG2细胞在裸鼠体内移植瘤生长,减轻瘤体体积和重量。结论miR-320a通过靶向抑制FOXQ1抑制肝癌细胞的增殖和侵袭。Objective To investigate the effect of miR-320a on the proliferation and invasion of hepatocellular carcinoma cells and its molecular mechanism.Methods Quantitative polymerase chain reaction(qPCR)was used to detect the expression levels of miR-320a in HepG2,SMMC-7721,BEL-7402,QGY-7701,QGY-7703 and HL-7702 cell lines.The effects of miR-320a on prolifera-tion,migration and invasion of HCC cells were detected by clonogenesis assay and transwell assay,respectively.Western blot was used to detect the expression levels of epithelial mesenchymal transition(EMT)related proteins including E-cadherin,N-cadherin,and vi-mentin.Dual luciferase reporter gene assay,qPCR,and Westem blot were used to verify the targeting effect of miR-320a on Forkhead protein Q1(FOXQ1).In vivo,the effect of overexpression of miR 320a on tumor forming ability of HCC cells was investigated by sub-cutaneous transplantation experiment in nude mice.Results The expression level of miR-320a was significantly down regulated in HCC cell lines.Overexpression of miR-320a can sigificantly inhibit the proliferation,migration and invasion,up-regulate the expres-sion of E-cadherin,down-regulate the expression of N-cadherin and vimentin,and inhibit EMT of HCC cells.MiR-320a inhibited the growth of transplanted tumors of HepG2 cells in nude mice by targeting FOXQ1 and reduced tumor volume and weight.Conclusion MiR-320a inhibited the proliferation and invasion of HCC cells by targeting FOXQ1.

关 键 词：miR-320a 肝细胞癌 叉头框蛋白Q1 增殖 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]