普兰林肽对5×FAD小鼠和WT小鼠认知行为及脑和视网膜β淀粉样蛋白6E10、炎症因子和神经细胞形态的影响  

Effects of pranlinide on cognitive behavior,βamyloid protein 6E10,inflammatory factors and neuronal cell morphology in brain and retina of 5×FAD mice and WT mice

作　　者：侯翌葳 杨宇 王博 段英涛 姚宏波[3] HOU Yi-wei;YANG Yu;WANG Bo;DUAN Ying-tao;YAO Hong-bo(Grade 2018,Basic Medical Science College,Qiqihar Medical University,Heilongjiang Qiqihaer 161006,China;Department of General Surgery,Xunke County People's Hospital,Heilongjiang Heihe 164499,China;Department of Histology and Embryology,Basic Medical Science College,Qiqihar Medical University,Heilongjiang Qiqihar 161006,China)

机构地区：[1]齐齐哈尔医学院基础医学院,黑龙江齐齐哈尔161006 [2]逊克县人民医院普外科,黑龙江黑河164499 [3]齐齐哈尔医学院基础医学院组织学胚胎学教研室,黑龙江齐齐哈尔161006

出　　处：《解剖学报》2023年第3期283-288,共6页Acta Anatomica Sinica

基　　金：黑龙江省教育厅基本科研业务费基础研究项目(2020-KYYWF-0012);黑龙江省自然科学基金(LH2021H122)。

摘　　要：目的 探讨普兰林肽对5×FAD小鼠和WT小鼠认知行为及脑和视网膜的β淀粉样蛋白(Aβ)6E10、炎症因子和神经细胞形态的影响。方法 实验选取5×FAD小鼠32只、WT小鼠16只,均为雌性。随机将5×FAD小鼠分为空白组、治疗组;WT组不进行任何处理;空白组腹腔注射PBS溶液;治疗组每天腹腔注射1次普兰林肽[200μg/(μ·d)],治疗8周。Morris水迷宫实验测定其认知行为的变化;采用免疫组织化学检测小鼠海马组织细胞、视网膜中Aβ6E10蛋白的表达;ELISA测定肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)炎症因子的含量。HE染色测定小鼠海马组织中神经细胞的排列和形态。结果 治疗组的潜伏时间短于5×FAD组,差异有统计学意义(P<0.05);治疗组的穿越平台次数和目标象限停留百分比高于5×FAD组,差异有统计学意义(P<0.05);5×FAD组脑内和视网膜内的Aβ6E10面积高于治疗组,差异具有统计学意义(P<0.05);视网膜和脑内的Aβ6E10具有正相关(P<0.05)。治疗组与5×FAD组脑中和视网膜中的TNF-α和IL-1β比较,差异无统计学意义(P>0.05)。治疗组较5×FAD组神经细胞排列规则,边界清晰。结论 普兰林肽可以改善小鼠的认知能力;使神经细胞排列较为规整,形态规则边界清晰;神经胶质细胞分布集中;周围间隙减小;脑内和视网膜内Aβ6E10具有同步性。Objective To study the effects of pranlinide on cognitive behavior,βamyloid(Aβ)protein 6E10,inflammatory factors and neuronal cell morphology in brain and retina of 5×FAD mice and WT mice.Methods Thirty two 5×FAD mice and 16 WT mice were selected.All were female 5×FAD mice were randomly divided into blank group and treatment group;No treatment was given in WT group.Blank group was intraperitoneally injected with PBS;treatment group was received intraperitoneal injection of pranlinide once a day for 8 weeks.The changes of cognitive ability were measured by Morris water maze test.The expression of Ap6E10 protein in mice hippocampal cells and retina was detected by immunohistochemistry.Tumor necrosis factor-α(TNF-α)was determined by enzyme-linked immunosorbent assay.The same method was also used for interleukin-1β(IL-1β)detection(the content of inflammatory factors).The arrangement and morphology of nerve cells in mouse hippocampal tissue were determined by hematoxylin-eosin(HE)staining.Results The latency time of treatment group was shorter than that of 5×FAD group,and the times of crossing the platform and the percentage of target quadrant stay in the treatment group were higher than those in the 5×FAD group,and the diferences were statistically significant(P<0.05).Compared with the retinal area and the brain area of Aβ6E10 in the 5×FAD group and in the treatment group,the content was halved(P<0.05).There was positive correlation between Aβ6E10 in retina and brain(P<0.05).There was no significant difference in TNF-αand IL-1βin brain and retina between treatment group and 5×FAD group(P>0.05).Compared with the 5×FAD group,the nerve cells in the treatment group were arramged in order and clear relatively.The distribution of glial cells was concentrated;The surrounding clearance was small.Conclusion Pranlinide can improve the cognitive ability of mice.The arrangement of nerve cells is regular,the shape is regular and the boundary is clear;The distribution of glial cells is concentrated;Surrounding of

关 键 词：普兰林肽 阿尔茨海默病 β淀粉样蛋白6E10 肿瘤坏死因子α 白细胞介素1Β 酶联免疫吸附测定 小鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]