拉萨河中氨氧化微生物群落特征及其影响因素分析  

Characteristics of Ammonia-oxidizing Microbial Communities in the Lhasa River and Its Influencing Factors

作　　者：王夏祎 潘永泰 刘贵华[3,4] 马琳 WANG Xiayi;PAN Yongtai;LIU Guihua;MALin(Research Center for Ecology and Environment of Qinghai-Tibetan Plateau,Tibet University,Lhasa 850000,Tibet Autonomous Region,P.R.China;College of Science,Tibet University,Lhasa 850000,Tibet Autonomous Region,P.R.China;Key Laboratory of Aquatic Botany and Watershed Ecology,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,Hubei,P.R.China;Hubei Key Laboratory of Wetland Evolution&Ecological Restoration,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,Hubei,P.R.China)

机构地区：[1]西藏大学青藏高原生态与环境研究中心,西藏拉萨850000 [2]西藏大学理学院,西藏拉萨850000 [3]中国科学院武汉植物园水生植物与流域生态重点实验室,湖北武汉430074 [4]中国科学院武汉植物园湿地演化与生态恢复湖北省重点实验室,湖北武汉430074

出　　处：《湿地科学》2023年第2期248-258,共11页Wetland Science

基　　金：国家自然科学基金项目(31971479)资助。

摘　　要：在拉萨河干流和支流水体中,设置了25个采样点;于2020年8月12日至26日,在各采样点水面下20 cm处,采集了河水样品;利用高通量测序技术,测定水体中氨氧化微生物群落的组成,计算出各菌种的相对丰度;采用Spearman相关分析方法和冗余分析方法,分析水环境因素对氨氧化微生物群落结构的影响。研究结果表明,在属水平上,水体中相对丰度最大的氨氧化古菌(ammonia-oxidizing archaea)为一种未被分类的奇古菌属(Thaumarchaeota),其在5个采样点水体中的相对丰度都大于50%;水体中相对丰度最大的氨氧化细菌(ammonia-oxidizing bacteria)为亚硝化螺菌属(Nitrosospira),其在4个采样点水体中的相对丰度都大于80%;水体中氨氧化古菌群落的Simpson多样性指数与年降水量显著负相关;各采样点水体中的氨氧化古菌和氨氧化细菌群落的相对丰度主要受水体中的总氮含量、总有机碳含量、硝态氮含量和水体的氧化还原电位、电导率以及太阳辐射强度影响。Twenty-five sampling sites were set up in main stream and tributary of the Lhasa River,and the water samples were collected at 20 cm below the water surface from 12 August to 26 August,2020.Highthroughput sequencing technology was used to determine the composition of ammonia-oxidizing microbial communities and the relative abundance of each species in water.Spearman correlation analysis and redundancy analysis methods were used to analyze the effects of environmental factors on the community structure of ammonia-oxidizing microorganisms.The results showed that at the genus level,unclassified Thaumarchaeota was the most abundant ammonia-oxidizing archaea in water,with its relative abundance reaching more than 50%in waters at five sampling sites.Nitrosospira was the most abundant ammoniaoxidizing bacteria in water,with its relative abundance reaching more than 80%in waters at four sampling sites.The Simpson index of the ammonia-oxidizing archaea community in the water was significantly negatively correlated with annual precipitation.The relative abundance of the ammonia-oxidizing microbial community in water was mainly influenced by the total nitrogen content,total organic carbon content,nitrate nitrogen content,oxidation reduction potential,electrical conductivity in water and solar radiation intensity.

关 键 词：氨氧化古菌 氨氧化细菌 硝化作用 冗余分析 拉萨河 

分 类 号：X172[环境科学与工程—环境科学]