腺苷对大鼠脑缺血再灌注后基质金属蛋白酶9和闭锁连接蛋白1表达的影响  

Effects of adenosine on MMP-9 and ZO-1 expression in cerebral ischemia-reperfusion rats

作　　者：刘坤[1] 吕欣 张先龙 赵莲辉 杨晓霞[1] Liu Kun;Lü Xin;Zhang Xianlong;Zhao Lianhui;Yang Xiaoxia(Rehabilitation School,Shenyang Medical College,Shenyang 110034,Liaoning Province,China)

机构地区：[1]沈阳医学院康复医学院,110034

出　　处：《中华老年心脑血管病杂志》2023年第5期528-531,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：辽宁省自然科学基金(20180550384);沈阳医学院大学生科研立项(20209017)。

摘　　要：目的观察腺苷预处理对大鼠脑缺血再灌注后基质金属蛋白酶9(MMP-9)和闭锁连接蛋白1(ZO-1)表达变化的影响。方法108只健康雄性SD大鼠随机分为对照组、模型组、腺苷组(n=36)。造模前腺苷组腹腔注射腺苷注射液,对照组和模型组大鼠在相同时间点腹腔注射2 ml生理盐水。采用改良的线栓法栓塞右侧大脑中动脉阻断血流2 h后,将线拔出形成再灌注。再灌注24 h后,伊文思蓝(EB)渗透法测定血脑屏障通透性,检测MMP-9和ZO-1表达。结果模型组和腺苷组EB,脑含水量,MMP-9明显高于对照组,ZO-1明显低于对照组(P<0.01);模型组和腺苷组MMP-9/β肌动蛋白(β-actin)表达明显高于对照组(0.563±0.054和0.377±0.080 vs 0.242±0.021,P<0.01),ZO-1/β-actin表达明显低于对照组(0.186±0.042和0.393±0.075 vs 0.671±0.065,P<0.01)。腺苷组MMP-9/β-actin表达低于模型组,ZO-1/β-actin表达明显高于模型组(P<0.01)。结论腺苷预处理可以通过抑制MMP-9表达,增加ZO-1表达,改善血脑屏障完整性,减轻脑水肿与脑缺血再灌注后的神经损伤。Objective To determine the effect of adenosine pretreatment on the expression of MMP-9 and ZO-1 in rats after cerebral ischemia-reperfusion(IR)injury.Methods A total of 108 healthy male SD rats were randomly divided into control,model and adenosine group(n=6).Before modeling,adenosine injection was intraperitoneally injected into the rats from the adenosine group,while 2 ml normal saline was given to the rats from the other 2 groups.The cerebral IR injury model was established with modified method by occlusion of right middle cerebral artery for 2 h followed by reperfusion of 24 h.Then,BBB permeability was assessed by Evans blue(EB)extravasation,the expression changes of MMP-9 and ZO-1 were measured.Results The model group and the adenosine group obtained significantly higher EB and brain water content,higher expression level of MMP-9,and lower level of ZO-1 when compared with the control group(P<0.01).The model group and the adenosine group obtained significantly higher expression of MMP-9/β-actin(0.563±0.054 and 0.377±0.080 vs 0.242±0.021,P<0.01),but lower expression of ZO-1/β-actin(0.186±0.042 and 0.393±0.075 vs 0.671±0.065,P<0.01)when compared with the control group.The adensine group obtained significantly lower expression of MMP-9/β-actin,but higher expression of ZO-1/β-actin when compared with the model group(P<0.01).Conclusion Adenosine pretreatment could inhibit MMP-9 and enhance ZO-1 expression to improve BBB integrity and alleviate cerebral edema,and thus alleviate nervous system injury.

关 键 词：腺苷 基质金属蛋白酶9 再灌注损伤 闭锁小带蛋白-1 

分 类 号：R743.3[医药卫生—神经病学与精神病学]