外泌体miR-338对骨质疏松大鼠骨代谢水平、骨小梁微结构和骨生物力学的影响  

Effects of Exosome MiR-338 on Bone Metabolism,TrabecularMicrostructure and Bone Biomechanics in Osteoporotic Rats

作　　者：张玉婷[1] 侯静雯 张蕾[1] 朱新华[1] 王枚[1] 许慧娟 张晓阳[1] ZHANG Yu-ting;HOU Jing-wen;ZHANG Lei;ZHU Xin-hua;WANG Mei;XU Hui-juan;ZHANG Xiao-yang(Department of Geriatrics,The Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang,830000,China)

机构地区：[1]新疆医科大学第五附属医院老年病科,新疆乌鲁木齐830000

出　　处：《现代生物医学进展》2023年第9期1631-1635,共5页Progress in Modern Biomedicine

基　　金：新疆维吾尔自治区自然科学基金项目(2021D01C451)。

摘　　要：目的:探讨外泌体miR-338对骨质疏松大鼠骨代谢水平、骨小梁微结构和骨生物力的影响。方法:采用健康成年SPF级SD雄性大鼠进行骨髓间充质干细胞(BMSCs)分离。采用双侧卵巢摘除手术方法构建了骨质疏松大鼠模型。采用qRT-PCR法检测miR-338的表达水平;检测大鼠的骨密度,骨小梁微结构和骨生物力学指标。结果:与空白对照组相比,OP模型组、OP+ExoBMSCs、抑制组和过表达组miR-338的表达水平明显更高(P<0.05);抑制组的miR-338的表达水平低于OP模型组、OP+ExoBMSCs和过表达组(P<0.05);与空白对照组相比,OP模型组、OP+ExoBMSCs、抑制组和过表达组OC、PINP、BALP的表达水平明显更低(P<0.05);抑制组的OC、PINP、BALP的表达水平明显高于OP模型组、OP+ExoBMSCs和过表达组(P<0.05);与空白对照组相比,OP模型组、OP+ExoBMSCs、抑制组和过表达组BV/TV、Th.N、Tb.Th、Conn.D水平更低,而Tb.Sp、SMI明显更高(P<0.05);抑制组组的BV/TV、Th.N、Tb.Th、Conn.D水平明显高于OP模型组、OP+ExoBMSCs和过表达组,而Tb.Sp、SMI更低(P<0.05);与空白对照组相比,OP模型组、OP+ExoBMSCs、抑制组和过表达组BMD、最大荷载、最大应力、最大位移、刚度水平更低(P<0.05);抑制组的BMD、最大荷载、最大应力、最大位移、刚度水平高于OP模型组、OP+ExoBMSCs和过表达组(P<0.05)。结论:BMSCs源性的miR-338可影响骨质疏松大鼠骨代谢、骨小梁微结构和骨生物力学状态。Objective:To investigate the effects of exosome miR-338 on bone metabolism,trabecular microstructure and bone biodynamics in osteoporosis rats.Methods:Bone marrow mesenchymal stem cells(BMSCs)were isolated from healthy adult SPF SD male rats.A rat model of osteoporosis was established by bilateral ovariectomy.The expression level of miR-338 was detected by qRT-PCR.Bone mineral density,trabecular microstructure and bone biomechanics were measured.Results:Compared with blank control group,the expression level of miR-338 was higher in OP model group,OP+ExoBMSCs,inhibition group and overexpression group(P<0.05).The expression level of miR-338 in inhibition group was lower than that in OP model group,OP+ExoBMSCs and overexpression group(P<0.05).Compared with blank control group,the expression levels of OC,PINP and BALP in OP model group,OP+ExoBMSCs,inhibition group and overexpression group were lower(P<0.05).The expression levels of OC,PINP and BALP in inhibition group were higher than those in OP model group,OP+ExoBMSCs and overexpression group(P<0.05).Compared with blank control group,the levels of BV/TV,Th.N,Tb.Th and Conn.D in OP model group,OP+ExoBMSCs,inhibition group and overexpression group were lower,Tb.Sp and SMI were higher(P<0.05).The levels of BV/TV,Th.N,Tb.Th and Conn.D in inhibition group were higher than those in OP model group,OP+ExoBMSCs and overexpression group(P<0.05),Tb.Sp and SMI were lower(P<0.05).Compared with blank control group,the levels of BMD,maximum load,maximum stress,maximum displacement and stiffness in OP model group,OP+ExoBMSCs,inhibition group and overexpression group were lower(P<0.05).The levels of BMD,maximum load,maximum stress,maximum displacement and stiffness in inhibition group were higher than those in OP model group,OP+ExoBMSCs and overexpression group(P<0.05).Conclusion:BMScS-derived miR-338 can affect bone metabolism,trabecular microstructure and bone biomechanical status in osteoporosis rats.

关 键 词：外泌体 miR-338 骨质疏松 大鼠 

分 类 号：R-33[医药卫生] R68