米蒸地黄辅料米的优选及其抗骨质疏松作用研究  

Adjuvant rice optimization of rice-steamed Rehmanniae Radix and its anti-osteoporosis effect

作　　者：杨红玉 张颖[2] 吴梦茜 朱慧[3] 李洪梅[4] 黄璐琦[2] 田慧[1] 袁媛 YANG Hong-yu;ZHANG Ying;WU Meng-xi;ZHU Hui;LI Hong-mei;HUANG Lu-qi;TIAN Hui;YUAN Yuan(Guangxi University of Chinese Medicine,Nanning 530200,China;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Nanjing University of Chinese Medicine,Nanjing 210023,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]广西中医药大学,广西南宁530200 [2]中国中医科学院中药资源中心,北京100700 [3]南京中医药大学,江苏南京210023 [4]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2023年第10期2749-2756,共8页China Journal of Chinese Materia Medica

基　　金：中央本级重大支减项目(2060302);中央级公益性科研院所基本科研业务费专项(ZZ10-008);中国中医科学院科技创新工程项目(CI2021A05205)。

摘　　要：该文以粳米、小米、黄米、黑米和糯米为原料,研究不同辅料米对米蒸地黄质量的影响,并选择最优辅料米蒸制地黄,对其抗骨质疏松作用进行分析。在建立梓醇和地黄苷D的UPLC-MS/MS含量测定方法的基础上,以梓醇及地黄苷D含量、性状评分、口感评分作为评价指标,采用综合加权评分法评价不同辅料米对米蒸地黄质量的影响,进行辅料米的优选。采用卵巢切除法建立骨质疏松大鼠模型,将SD大鼠随机分为假手术组,模型组,阳性药组,生地黄、米蒸地黄、清蒸地黄、淫羊藿-米蒸地黄的高剂量组和低剂量组;连续给药12周后,测定大鼠体质量、骨钙含量和骨密度。结果表明,以粳米为辅料的米蒸地黄的综合评分最高,因此选择粳米为最佳辅料米蒸制地黄;生地黄、米蒸地黄、清蒸地黄、淫羊藿-米蒸地黄给药后均可通过提高骨钙含量、增加骨密度改善骨质疏松;与生地黄相比,米蒸地黄改善骨质疏松的效果更佳,且米蒸地黄与清蒸地黄相比无显著性差异。该研究明确了粳米为米蒸地黄的最佳辅料米,并证实了米蒸地黄具有改善骨质疏松的作用,为进一步研究其药理作用和机制奠定了基础。The present study aimed to investigate the effect of various adjuvant rice on the quality of rice-steamed Rehmanniae Radix(RSRR)with Japonica rice,millet,yellow rice,black rice,and glutinous rice as raw materials,and analyze the anti-osteoporosis effect of RSRR by the optimal adjuvant rice.On the basis of the established UPLC-MS/MS method for the determination of the content of catalpol and rehmannioside D,comprehensive weighted scoring method was employed to evaluate the effect of various auxiliary rice on the quality of RSRR with the content of catalpol and rehmannioside D,character score,and taste score as indicators to optimize adjuvant rice.The osteoporosis model was induced by ovariectomy in rats.SD rats were randomly divided into a sham operation group,a model group,a positive control group,and low-dose and high-dose groups of Rehmanniae Radix,RSRR,steamed Rehmanniae Radix,and Epimedii Folium-RSRR.After treatment for 12 weeks,body weight,bone calcium content,and bone mineral density were measured.The results showed that Japonica rice was selected as the optimal adjuvant due to the highest comprehensive score of RSRR steamed by Japonica rice.Rehmanniae Radix,RSRR,steamed Rehmanniae Radix,as well as Epimedii Folium-RSRR,could improve osteoporosis by increasing bone calcium content and bone mineral density.RSRR was superior to Rehmanniae Radix in improving osteo-porosis.However,there was no significant difference between RSRR and steamed Rehmanniae Radix.This study confirmed that Japonica rice was the optimal adjuvant rice of RSRR and verified the anti-osteoporosis effect of RSRR,which laid a foundation for further research on the pharmacological action and mechanism of RSRR.

关 键 词：米蒸地黄 粳米 骨质疏松 去卵巢大鼠 

分 类 号：R285.5[医药卫生—中药学]