An integrated microfluidic chip-mass spectrometry system for rapid antimicrobial resistance analysis of bacteria producingβ-lactamases  

作　　者：Zhaochen Su Wanting Hu Lizhen Ye Dan Gao Jin-Ming Lin 

机构地区：[1]State Key Laboratory of Chemical Oncogenomics,Key Laboratory of Chemical Biology,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen 518055,China [2]Department of Chemistry,Beijing Key Laboratory of Microanalytical Methods and Instrumentation,Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical Biology(Ministry of Education),Tsinghua University,Beijing 100084,China

出　　处：《Chinese Chemical Letters》2023年第5期323-326,共4页中国化学快报（英文版）

基　　金：supported by Research and Development Program in Key Areas of Guangdong Province,China(No.2019B020209009);Natural Science Foundation of Guangdong Province,China(Nos.2020A1515010660 and 2022A1515011437);Shenzhen Fundamental Research and Discipline Layout project(No.JCYJ20180508152244835)。

摘　　要：Bacteria producingβ-lactamases have become a major issue in the global public health field.To restrain the development of drug resistance and reduce the abuse of antibiotics,it is very important to rapidly identify bacteria producingβ-lactamases and put forward a reasonable treatment plan.Here,an integrated microfluidic chip-mass spectrometry system was proposed for rapid screening ofβ-lactamaseproducing bacteria and optimization ofβ-lactamase inhibitor dosing concentration.The concentration gradient generator followed by an array of bacterial culture chambers,as well as micro-solid-phase extraction columns was designed for sample pretreatment before mass analysis.By using the combination system,the process of the hydrolysis of antibiotics byβ-lactamase-producing bacteria could be analyzed.To validate the feasibility,four antibiotics and two antibiotic inhibitors were investigated using three strains including negative control,SHV-1 and TEM-1 strains.SHV-1 and TEM-1 strains were successfully distinguished as theβ-lactamase producing strains.And the acquired optimal concentrations ofβ-lactamase inhibitors were in accordance with the results by that obtained from the traditional microdilution broth method.The total analysis time only needed around 2 h,which was faster than conventional methods that require a few days.The technique presented herein provides an easy and rapid protocol forβ-lactamase resistance related studies,which is important for the inhibition of antimicrobial resistance development and the reduction of antibiotics abuse.

关 键 词：MICROFLUIDICS Mass spectrometry Β-LACTAMASES Antimicrobial resistance INHIBITOR 

分 类 号：R446.5[医药卫生—诊断学] TH843[医药卫生—临床医学]