Diagnosis and subtype classification on serum peptide fingerprints by mesoporous polydopamine with built-in metal-organic framework  

作　　者：Zixing Xu Haolin Chen Huimin Chu Xizhong Shen Chunhui Deng Nianrong Sun Hao Wu 

机构地区：[1]Department of Gastroenterology and Hepatology,Zhongshan Hospital,Department of Chemistry,Institute of Metabolism&Integrate Biology(IMIB),Fudan University,Shanghai 200433,China [2]Department of Gastroenterology and Hepatology,Zhongshan Hospital,Fudan University,Shanghai 200032,China

出　　处：《Chinese Chemical Letters》2023年第5期352-356,共5页中国化学快报（英文版）

基　　金：financially supported by National Key R&D Program of China(No.2018YFA0507501);the National Natural Science Foundation of China(Nos.22074019,21425518,22004017);Shanghai Sailing Program(No.20YF1405300)。

摘　　要：Crohn's disease(CD)as a big issue to public health needs an accurate diagnosis urgently that is the common challenge among internal diseases.Herein,we design a mesoporous polydopamine with builtin metal-organic frameworks(dubbed MMP-b-MOFs)to combine with high-throughput mass spectrometry to extract serum peptide fingerprints from CD and healthy controls(HC).Benefitting by the sizeexclusion and strong hydrophilicity of MMP-b-MOFs,the extracted peptide fingerprints present extremely high quality.CD and HC are explicitly discriminated with orthogonal partial least squares discriminant analysis(OPLS-DA),the corresponding area under the curve(AUC)value is 1.000.Moreover,eight peptides with clear identity are screened out and achieve the accurate diagnosis and subtype classification of CD,with all AUC values up to 1.000.Moreover,the unsupervised model is also established to precisely classify HC and CD based on these eight clearly identified peptides.This work brings great benefits for clinical detection especially internal medicine.

关 键 词：Mesoporous dopamine Metal-organic frameworks Serum peptides Mass spectrometry Crohn’s disease Disease diagnosis 

分 类 号：TQ317[化学工程—高聚物工业] R574[医药卫生—消化系统]