大豆品种齐黄34广适性的遗传分析  

Genetic Analysis of Wide Adaptability of Soybean Cultivar Qihuang 34

作　　者：刘薇 王玉斌 李伟[1] 张礼凤[1] 王彩洁[1] 徐冉[1] 戴海英[1] 张彦威[1] Liu Wei;Wang Yubin;Li Wei;Zhang Lifeng;Wang Caijie;Xu Ran;Dai Haiying;Zhang Yanwei(Crop Research Institute,Shandong Academy of Agricultural Sciences/Shandong Engineering Laboratory of Featured Crops,Jinan 250100,China)

机构地区：[1]山东省农业科学院作物研究所/山东省特色作物工程实验室,山东济南250100

出　　处：《山东农业科学》2023年第5期22-27,共6页Shandong Agricultural Sciences

基　　金：山东省重点研发计划(重大科技创新工程)项目(2021LZGC025);山东省农业良种工程项目(2021LZGC003);山东省自然科学基金项目(ZR2020QC119)。

摘　　要：为了阐释齐黄34广适性的遗传机理,本研究利用齐黄34与冀豆17杂交衍生的重组自交系群体进行开花期的QTL定位,得到1个位于6号染色体的QTL位点。该位点在两种环境下稳定存在,表型贡献率为33.93%~40.04%。该QTL中包含重要的生育期基因E1,其在齐黄34中为显性基因型,而在冀豆17中为功能部分缺失的e1-as基因型。qRT-PCR表明,受E1基因调控的开花促进基因GmFT2a在齐黄34中的表达量低于冀豆17,且该基因的累积滞后于冀豆17,造成齐黄34开花期晚于冀豆17。因此,显性E1基因型的存在可能是齐黄34能够适应低纬度地区的一个重要原因。本研究为阐明齐黄34适应范围广的遗传机理提供了一定的分子基础,同时为齐黄34的遗传改良提供了分子依据。In order to analyze the genetic mechanism of wide adaptability of soybean Qihuang 34,we used the recombinant inbred line population derived from the cross of Qihuang 34 and Jidou 17 to conduct the QTL analysis of flowering time.A QTL locus on chromosome 6 was obtained,which was stable in the two envi⁃ronments with phenotypic contribution rate as 33.93%~40.04%.This QTL contained an important gene E1 re⁃lated to growth period,which was dominant in Qihuang 34 but as a partially functional deletion e1⁃as genotype in Jidou 17.qRT⁃PCR showed that the expression level of flowering⁃promoting gene GmFT2a regulated by E1 gene was lower in Qihuang 34 compared with Jidou 17,and the accumulation of this gene in Qihuang 34 lagged behind that in Jidou 17,resulting in the flowering time of Qihuang 34 later than that of Jidou 17.There⁃fore,the dominant E1 genotype might be an important reason for the adaptation to low latitudes of Qihuang 34.These results could provide a certain molecular bases for elucidating the genetic mechanism of the wide adapta⁃tion of Qihuang 34,and also provide molecular bases for genetic improvement of Qihuang 34.

关 键 词：大豆 齐黄34 广适性 开花期 QTL E1基因 

分 类 号：S565.101[农业科学—作物学]