不同方法制备的肌醇对建鲤幼鱼生长性能、生理和肠道炎症应答的影响  

Effects of myo-inositol by different preparation methods on growth performance,physiology and intestinal inflammation related genes expressions in Cyprinus carpio var.jian

作　　者：罗晨皓 缪凌鸿[1,2] 林艳[2] 刘波[1,2] 戈贤平[1,2] LUO Chenhao;MIAO Linghong;LIN Yan;LIU Bo;GE Xianping(Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture and Rural Affairs,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China)

机构地区：[1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心,农业农村部淡水渔业和种质资源利用重点实验室,江苏无锡214081

出　　处：《水产学报》2023年第6期51-61,共11页Journal of Fisheries of China

基　　金：现代农业产业技术体系国家大宗淡水鱼产业技术体系(CARS-45)。

摘　　要：为了探讨不同方法制备的肌醇及其添加量对建鲤生长性能、生理生化以及抗氧化能力的影响,实验以蛋白含量34.64%和脂肪含量7.86%制备基础日粮(C),通过在基础日粮中分别添加400 mg/kg酶促法肌醇(C+400E-MI)和400 mg/kg化工法肌醇(C+400C-MI)配制3种实验日粮,选取当年繁育的健康的建鲤幼鱼[(1.5±0.01) g]进行为期10周的养殖实验。养殖结束后,测定其生长性能、肝脏抗氧化酶活性和炎症相关基因的表达。结果显示,与对照组相比,基础日粮中添加不同制备方法的肌醇均具有提高建鲤幼鱼的特定生长率、蛋白质效率和蛋白质沉积率的作用;能降低血浆中乳酸脱氢酶的含量,有效保护肝细胞。与对照组相比,添加400 mg/kg酶促法肌醇可以显著提高建鲤幼鱼肝脏总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物酶(GPX)活性,增加谷胱甘肽(GSH)的含量。添加400mg/kg化工法肌醇能够显著提高建鲤幼鱼肝脏GPX的活性,但同时也显著提高了丙二醛(MDA)的含量。进一步对基因表达的分析发现,2种肌醇的添加均显著降低了建鲤幼鱼肠道中促炎因子IL-6、TNF-α和IL-12在mRNA水平的表达,同时,添加400 mg/kg酶促法肌醇还显著降低了肠道中抗炎因子TGF-β1和IL-10在mRNA水平的表达。总体而言,饲料中添加400 mg/kg化工法和酶促法制备获得的肌醇均可以提高建鲤幼鱼的生长和饲料利用,抑制了肠道炎症反应,而酶促法肌醇对建鲤幼鱼的生长促进和抗氧化能力增强效果更优,因此,酶促法肌醇是一种符合绿色发展要求的水产动物营养添加剂。本实验可为不同方法制备的肌醇作为饲料添加剂在水产养殖中的应用提供参考依据。This study was conducted to investigate the effects of myo-inositol by different preparation methods on growth performance,plasma biochemical indicators,hepatic antioxidant capability and intestinal anti-inflammatory gene expressions of Cyprinus carpio var.jian.A basic diet(C,34.64%of crude protein and 7.86%of crude fat)was formulated,and two experimental diets 400 mg/kg enzymatic myo-inositol(C+400E-MI)and chemical myo-inositol(C+400C-MI)in the basic diet were prepared.180 healthy juvenile C.carpio var.jian[(1.50±0.01)g]were randomly distributed to 9 tanks in triplicate at the density of 20 fish per tank.After the 10-week feeding trial,the growth performance,liver antioxidant ability and intestinal inflammatory response-related gene expressions were determined.The results showed that the dietary E-MI and C-MI increased protein efficiency and protein deposition rate(P<0.05),and 400 mg/kg of dietary E-MI improved the weight gain rate and specific growth rate compared to the control group(P<0.05),and reduced the content of plasma lactate dehydrogenase(P<0.05).Compared to the basic group,400 mg/kg E-MI supplementation significantly increased the activities of hepatic total superoxide dismutase(T-SOD),glutathione(GSH)and glutathione peroxidase(GPX)(P<0.05).400 mg/kg C-MI supplementation significantly increased the hepatic GPX(P<0.05),while the content of malondialdehyde(MDA)increased as well(P<0.05).The intestinal gene expressions showed that the inclusion of E-MI and C-MI significantly down-regulated the mRNA expressions of pro-inflammatory factors in the fish intestine,e.g.IL-6,TNF-αand IL-12(P<0.05).The inclusion of 400 mg/kg E-MI significantly reduced the mRNA expressions of anti-inflammatory factors,e.g.TGF-β1 and il-10(P<0.05).In general,the supplementation of 400 mg/kg myo-inositol by different preparation methods improved the growth and feed utilization of juvenile C.carpio var.jian,and inhibited inflammatory response in the intestine.In addition,the enzymatic myo-inositol exhibited a better effect on grow

关 键 词：建鲤 肌醇 制备方法 生长性能 抗氧化能力 炎症因子 

分 类 号：S963.73[农业科学—水产养殖]