一株产灵菌红素菌株黏质沙雷氏菌H04的分离鉴定及发酵条件优化  

Isolation,identification,and optimization of fermentation conditions of a high-yielding prodigiosin Serratia marcescens H04

作　　者：王鹏宇 徐晨琪 彭涵 姜宏年 李翯 霍焱焜 任虹[1] WANG Pengyu;XU Chenqi;PENG Han;JIANG Hongnian;LI He;HUO Yankun;REN Hong(School of Light Industry,Beijing Technology and Business University,Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry,Beijing 100048,Chnia)

机构地区：[1]北京工商大学轻工科学技术学院,中国轻工业清洁生产和资源综合利用重点实验室,北京100048

出　　处：《食品与发酵工业》2023年第11期20-26,共7页Food and Fermentation Industries

基　　金：“十三五”国家重点研发计划课题项目(2016YFD0400802)。

摘　　要：灵菌红素具有抗氧化、抗肿瘤等生物活性和潜在的医用价值,大规模生产对其研究和开发具有重要意义。从槐树根际土壤中筛选出1株产红色素菌株H04,根据16S rDNA系统发育树鉴定该菌株为黏质沙雷氏菌Serratia marcesence。其合成的红色素经UV、HPLC-MS分析,确定为灵菌红素。利用单因素试验和正交试验优化发酵条件,以提高色素产量。最佳发酵条件为:培养基使用15 g/L蔗糖为碳源、0.5 g/L酵母浸粉和0.5 g/L(NH_(4))_(2)SO_(4)为氮源、5 g/L NaCl、0.75 g/L KCl、10 g/L脯氨酸、0.5 g/L CaCO_(3);接种量3%(体积分数)、温度25℃、转速180 r/min、培养时间24 h。优化后发酵得灵菌红素提取物4.5 g/L,与正交优化前相比产量提高了2.3倍。The large-scale production of prodigiosin,which has biological activities such as antioxidant and antitumor,is of great importance for its research and development.A red pigment producing bacteria H04,isolated from the rhizosphere of Sophora japonica Linn.,was identified as Serratia marcescens according to the 16S rDNA phylogenetic tree.The red pigment derived from H04 was determined as prodigiosin through UV and HPLC-MS.Single-factor and orthogonal experiments were used to optimize the fermentation conditions to improve the pigment yield.The optimum conditions were the fermentation medium using 15 g/L sucrose as carbon source,0.5 g/L yeast extract powder,and 0.5 g/L ammonium sulfate as nitrogen source,5 g/L NaCl,0.75 g/L KCl,10 g/L proline,and 0.5 g/L CaCO_(3);inoculum amount was 3%,25℃,180 r/min,24 h.The optimized fermentation yielded 4.5 g/L of red pigment extract,with a 2.3-fold increase in yield compared to that before orthogonal optimization.

关 键 词：灵菌红素 黏质沙雷氏菌 系统发育分析 正交试验 优化发酵条件 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]