乳酸菌发酵中药对高脂血症模型小鼠调脂作用的初步探究  

Preliminary study on lipid-regulating effect of lactic acid bacteria fermented traditional Chinese medicine on hyperlipidemia model mice

作　　者：杜莹瑞 李市场[1] 陈妍 胡锋阳 丁慧 龚明贵[1] DU Yingrui;LI Shichang;CHEN Yan;HU Fengyang;DING Hui;GONG Minggui(School of Food and Biological Engineering,Henan University of Science and Technology,Luoyang 471023,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023

出　　处：《食品与发酵工业》2023年第11期55-61,共7页Food and Fermentation Industries

基　　金：国家自然科学基金项目(3187003)。

摘　　要：以山楂、决明子、葛根等混合植物为底物,通过发酵研究其成分分析、降脂作用、抗氧化作用和保肝作用。试验采用高效液相色谱串联质谱法对其成分进行定性定量分析,饲喂小鼠高脂饲料进行造模,造模成功后,给予不同剂量的发酵液和辛伐他汀,持续灌喂治疗4周,比较各组的总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)浓度、超氧化物歧化酶(superoxide dismutase,SOD)活力、丙二醛(malondialdehyde,MDA)含量、谷丙转氨酶(alanine transaminase,ALT)和谷草转氨酶(aspartate transaminase,AST)活力,比较各组的肝肾组织病理结果并进行分析。结果显示,灌胃4周后,与高脂模型组相比,发酵液各剂量组小鼠体质量极显著恢复(P<0.01)。与空白对照组相比,高脂模型组TC、TG浓度极显著升高(P<0.01)。模型组小鼠给予发酵液不同剂量治疗后,TG、TC、LDL-C浓度均极显著降低(P<0.01),HDL-C浓度极显著增加(P<0.01);SOD活性极显著升高(P<0.01),MDA含量、AST和ALT活力极显著降低(P<0.01)。肝组织病理结果表明发酵液可以消除脂肪粒,并随着发酵液浓度的增加效果也越明显;肾组织病理结果表明发酵液毒性较小,且高脂模型肾脏耐受性较强,对肾脏没有明显损伤。辛伐他汀的效果也比较明显,但不如发酵液。由此得出该发酵液具有一定的调节血脂、抗氧化和保肝作用,能较好地恢复高脂血症小鼠的脂肪肝,且具有量效关系,其降血脂作用优于辛伐他汀。To study the hypolipidemic,antioxidant and hepatoprotective effects of fermentation broth made from hawthorn,Cassia tora,and Pueraria lobata,HPLC-MS/MS was used for qualitative and quantitative analysis of the components of broth.Mice were fed a high-fat diet for modeling,and after successful modeling,different doses of fermentation broth(treatment group)and simvastatin(positive control)were administered by gavage for 4 weeks.The concentration of total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C)and malondialdehyde(MDA);the activity of superoxide dismutase(SOD),alanine transaminase(ALT)and aspartate transaminase(AST)were compared.The pathological results of liver and kidney tissues were compared and analyzed.The results showed that after 4 weeks of intragastric administration,compared with the high-fat model group,the body weight of mice in each treatment group recovered significantly(P<0.01).Compared with blank control,the concentrations of TC and TG in high-fat model group significantly increased(P<0.01).The concentrations of TG,TC,and LDL-C in treatment group significantly decreased(P<0.01),while that of HDL-C significantly increased(P<0.01).The activity of SOD was significantly increased(P<0.01),while the content of MDA,the activity of AST and ALT significantly decreased(P<0.01).Fermentation broth eliminated fat particles in live with a dose-dependent effect.Fermentation broth was less toxic to kidney.Kidneys of the high-fat model were more tolerant,with no significant damage to kidneys.The positive control group was also more effective,but not as effective as the fermentation broth.The fermentation broth has certain lipid-regulating,antioxidant,and hepatoprotective effects,and can better restore the fatty liver of hyperlipidemic mice,with has a quantitative-effect relationship,and its hypolipidemic effect is better than that of the positive control.

关 键 词：发酵液 成分分析 高脂血症 辛伐他汀 脂肪堆积 

分 类 号：R285.5[医药卫生—中药学]