豌豆蛋白水解物体外及对HepG2细胞的抗氧化活性  

Antioxidant activity of pea protein hydrolysates in vitro and on HepG2 cells

作　　者：赵丹[1] 刘晓兰 郑喜群 樊磊 ZHAO Dan;LIU Xiaolan;ZHENG Xiqun;FAN Lei(Key Laboratory of Food Science and Engineering,Harbin University of Commerce,Harbin 150028,China;Heilongjiang Key Laboratory of Corn Deep Processing Theory and Technology,Qiqihar University,Qiqihar 161006,China;College of Food Science in Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]哈尔滨商业大学食品工程学院,省高校食品科学与工程重点实验室,黑龙江哈尔滨150028 [2]齐齐哈尔大学食品与生物工程学院,黑龙江省玉米深加工理论与技术重点实验室,黑龙江齐齐哈尔161006 [3]黑龙江八一农垦大学食品学院,黑龙江大庆163319

出　　处：《食品与发酵工业》2023年第11期170-177,共8页Food and Fermentation Industries

基　　金：科技部“十三五”国家重点研究计划项目(2017YFD0400200);中央支持地方高校改革发展资金高水平人才项目(2020GSP08);哈尔滨商业大学研究生创新基金项目(YJSCX2018-483HSD)。

摘　　要：为了全面评价豌豆蛋白水解物(pea protein hydrolysates,PPH)及其超滤分离组分的体外及细胞抗氧化能力,以豌豆蛋白为原料,采用碱性蛋白酶和复合蛋白酶协同水解制备PPH,然后超滤分级得到PPH1(分子质量<3 kDa)、PPH2(分子质量3~5 kDa)、PPH3(分子质量>5 kDa)3个组分,使用不同的化学方法研究PPH和超滤分离组分的抗氧化活性,从中选择抗氧化能力最高的组分进一步考察其细胞内自由基清除能力并用H_(2)O_(2)应激的HepG2细胞评估其对氧化应激的保护作用。结果表明,各组分中PPH1具有最高的体外化学抗氧化活性,其氨基酸组成分析表明,抗氧化功能氨基酸占比高;在实验设定浓度下,PPH1能够显著抑制H_(2)O_(2)应激损伤HepG2细胞内活性氧的积累,对细胞应激损伤有明显的保护作用。研究认为,PPH及其超滤分离组分具有良好的体外化学及细胞抗氧化活性,对H_(2)O_(2)应激的HepG2细胞具有保护作用,能够为其作为一种天然源抗氧化剂提供依据。In this research,the antioxidant capacity of pea protein hydrolysates(PPH)and its fractions separated by ultrafiltration membrane were comprehensively analyzed in vitro and cells level.Using pea protein as raw material,pea protein hydrolysates were prepared by Alcalase and Protamex protases and then separated into three different molecular weight components by ultrafiltration:PPH1(<3 kDa),PPH2(3-5 kDa),PPH3(>5 kDa).The components with the highest antioxidant capacity were selected to further investigate their intracellular free radical scavenging ability and the protective effect against oxidative stress in H_(2) O_(2)-stressed HepG2 cells.The results showed that among the pea protein hydrolysates and the three components of the pea protein hydrolysates obtained by ultrafiltration,PPH1 had the best antioxidant activity in vitro,and amino acid composition had a high proportion of antioxidant functions amino acids.At the experimentally set of PPH1 could significantly inhibit the accumulation of ROS in HepG2 cells injured by H_(2) O_(2) stress.The pea protein hydrolysates and its fractions separated by ultrafiltration membrane possess good in vitro antioxidant ability and obvious protective effects on HepG2 cells from oxidative stress injury induced by H_(2) O_(2),which can provide a basis for its use as a natural source of antioxidants.

关 键 词：豌豆蛋白水解物 抗氧化 HEPG2细胞 氧化应激 

分 类 号：TS202.3[轻工技术与工程—食品科学]