苯丙氨酸脱氢酶分离纯化的开放实验教学设计与实践  

Design and practice of open experiment teaching for separation and purification of phenylalanine dehydrogenase

作　　者：李进军[1] 赵晨[1] 王辂[1] 李端华[1] Li Jinjun;Zhao Chen;Wang Lu;Li Duanhua(Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province,Department of Pharmacy,Chengdu University,Chengdu 610052,China)

机构地区：[1]成都大学药学院抗生素研究与再评价四川省重点实验室,四川成都610052

出　　处：《现代盐化工》2023年第2期114-116,共3页Modern Salt and Chemical Industry

基　　金：四川省科技厅国际合作项目“针对新型污染物-耐药基因的酶法清除技术研究”(2020YFH0013);四川省科技厅国际合作项目“基于噬菌体技术的皮炎湿疹新治疗方案研究”(2021YFH0016);成都市科技局国际合作项目“酶法清除耐药基因的关键技术研究”(2020-GH02-00031-HZ)。

摘　　要：开放实验教学既是一种教学理念,又是一种教学策略,在本科生毕业阶段和向研究生过渡的阶段显得尤为重要。本次开放实验教学设计依托课题组研究较为深入的苯丙氨酸脱氢酶,将发酵、提取、粗纯、精纯、酶活测定、高效液相色谱(HPLC)分析和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析的整条项目链作为教学和实践内容,同时训练学生整理、处理数据和撰写报告的能力,提升其综合科研水平。Open experiment teaching is not only a teaching philosophy,but also a teaching strategy,especially important at the undergraduate graduation stage and the transition stage to graduate students.This open experiment teaching design relies on the phenylalanine dehydrogenase that my research group has studied more deeply,and uses the whole project chain including fermentation,extraction,crude purification,fine purification,enzyme activity determination,high performance liquid chromatography(HPLC)analysis,and sodium dodecyl sulfate-polyacrylamide gelelectrophoresis(SDS-PAGE)analysis as teaching and practice content.At the same time,it trains students’ability to organize and process data and write reports,improving their comprehensive scientific research level.

关 键 词：苯丙氨酸脱氢酶 纯化 开放实验 教学设计与实践 

分 类 号：G642.423[文化科学—高等教育学] O629.8-4[文化科学—教育学]