人接头蛋白LNK对非小细胞肺癌细胞增殖和侵袭能力的影响  

Effects of human adaptor protein LNK on proliferation and invasion ability of non-small cell lung cancer cells

作　　者：王杰[1,2] 谷婷 宋吉欣 吴吟秋 戴春 戴华[1,2] WANG Jie;GU Ting;SONG Jixin;WU Yinqiu;DAI Chun;DAI Hua(Medical College of Yangzhou University,Yangzhou,Jiangsu,225001;Jiangsu Provincial Key Laboratory of Basic and Clinical Transformation of Non-Coding RNA,Yangzhou,Jiangsu,225001;Department of General Surgery,Yangzhong People′s Hospital of Jiangsu Province,Yangzhong,Jiangsu,212200)

机构地区：[1]扬州大学医学院,江苏扬州225001 [2]江苏省非编码RNA基础与临床转化重点实验室,江苏扬州225001 [3]江苏省扬中市人民医院普外科,江苏扬中212200

出　　处：《实用临床医药杂志》2023年第10期31-35,共5页Journal of Clinical Medicine in Practice

基　　金：国家自然科学基金资助项目(81472815);江苏省高校自然科学研究重大项目(20KJA320005);江苏省中西医结合老年病防治重点实验室开放课题(202209)。

摘　　要：目的探讨人接头蛋白LNK(hLNK)对非小细胞肺癌(NSCLC)发生发展的影响。方法构建稳定过表达hLNK的人NSCLC细胞株H1299-hLNK及对照细胞株H1299-pCDH。采用Western blot验证上述稳转细胞株中hLNK的表达水平;采用平板克隆实验分析hLNK过表达对H1299细胞增殖的影响;采用划痕实验分析hLNK过表达对H1299细胞侵袭能力的影响;对细胞中上皮间质转化(EMT)的典型标志蛋白进行分析。结果成功构建过表达hLNK的人NSCLC细胞株H1299-hLNK。与对照细胞H1299-pCDH比较,hLNK过表达抑制了H1299细胞的增殖和迁移能力,且细胞EMT水平被抑制,差异有统计学意义(P<0.05)。结论hLNK主要通过下调NSCLC细胞H1299的EMT水平抑制癌细胞的增殖、迁移。Objective To investigate the effect of human adaptor protein LNK(hLNK)on the occurrence and development of non-small cell lung cancer(NSCLC).Methods Human NSCLC cell line H1299-hLNK with stable overexpression of hLNK and control cell line H1299-pCDH were constructed.Western blot was used to verify the expression level of hLNK in the above stable cell lines;the effect of hLNK overexpression on the proliferation of H1299 cells was analyzed by plate cloning;the effect of hLNK overexpression on the invasion ability of H1299 cells was analyzed by scratch assay;the typical marker proteins of epithelial interstitial transition(EMT)in cells were analyzed.Results Human NSCLC cell line H1299-hLNK overexpressed with hLNK was successfully constructed.Compared with control cells H1299-pCDH,hLNK overexpression significantly inhibited the proliferation and migration of H1299 cells,and the EMT level of H1299 cells was significantly inhibited(P<0.05).Conclusion The hLNK can inhibit the proliferation and migration of cancer cells mainly by down-regulating the EMT level of NSCLC cell H1299.

关 键 词：接头蛋白LNK 非小细胞肺癌 H1299细胞系 增殖 迁移 上皮间质转化 

分 类 号：R734.2[医药卫生—肿瘤] R363.2[医药卫生—临床医学]