90个苹果品种耐寒性评价和全基因组关联分析  

Evaluation of Cold Tolerance of 90 Apple Cultivars and Genome Wide Association Analysis

作　　者：坎智勇 张德辉 李中兴 余思思 钱谦 樊天乐 李雪薇[1] 马锋旺[1] 管清美[1] KAN Zhiyong;ZHANG Dehui;LI Zhongxing;YU Sisi;QIAN Qian;FAN Tianle;LI Xuewei;MA Fengwang;GUAN Qingmei(State Key Laboratory of Crop Stress Biology for Arid Areas,College of Horticulture,Northwest A&F University,Yangling,Shannxi 712100,China)

机构地区：[1]西北农林科技大学园艺学院,旱区作物逆境生物学国家重点实验室,陕西杨凌712100

出　　处：《园艺学报》2023年第5期921-932,共12页Acta Horticulturae Sinica

基　　金：陕西省科技重大专项(2020zdzx03-01-02)。

摘　　要：以90个苹果栽培品种为试验材料,采集冬季休眠的枝条进行相对电导率测定,评价其耐寒能力;同时利用基因组重测序技术检测到的1247162个SNP开展全基因组关联分析,筛选与苹果耐寒性状显著关联的候选基因。鉴定出3个耐寒能力最强的品种,其相对电导率均小于40%,分别是‘金红’(‘Jinhong’),‘埃德尔博斯多夫’(‘Edelborsdorfer’),‘赫拉森’(‘Haralson’)。基于1247162个高质量SNP位点与表征耐寒性的相对电导率的指标的关联分析,挖掘出5个与耐寒性显著关联的SNP位点,这些位点分布在4号、15号和16号染色体上,并鉴定到与冷胁迫紧密相关的3个候选基因TIF3B1(MD04G1241100)、COR47(MD15G1003900)和MD16G1069900。In this study,ninety apple cultivars were used as experimental materials to evaluate cold tolerance by a relative electrical conductivity test.Genome-wide association analysis was conducted on 1247162 SNPs detected in apple accessions by genome resequencing technology,and candidate genes significantly associated with cold tolerance were selected.Three cultivars with the strongest cold tolerance were identified,and their relative electrical conductivity was all less than 40%,which were‘Jinhong',‘Ederbostdorf'and‘Haralson'.Based on the association analysis using 1247162 high-quality SNPs and index of relative electrical conductivity characterizing cold tolerance,five significant correlated loci related to cold tolerance were identified,which were distributed on chromosome 4,15,and 16,respectively.Three candidate genes TIF3B1(MD04G1241100),COR47(MD15G1003900),and MD16G1069900closely related to cold stress tolerance were identified.

关 键 词：苹果 耐寒能力 全基因组关联分析 

分 类 号：S661.1[农业科学—果树学]