牛奶中6种喹诺酮类兽药残留的QuEchERS-高效液相色谱-串联质谱测定法  

Determination of six quinolones residues in milk by QuEchERS-high-performance liquid chromatography-tandem mass spectrometry

作　　者：肖潇[1] 王冉冉[1] 艾依热提·买买提 李菁[1] 杨雪丽[1] 李方[1] XIAO Xiao;WANG Ran-ran;Aiyireti·Maimaiti;LI Jing;YANG Xue-li;LI Fang(Health Monitoring and Inspection Center,Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region,Urumqi Xinjiang,830002,China)

机构地区：[1]新疆维吾尔自治区疾病预防控制中心卫生监测检验中心,新疆乌鲁木齐830002

出　　处：《职业与健康》2023年第8期1046-1049,1056,共5页Occupation and Health

基　　金：新疆维吾尔自治区自然科学基金(2020D01C083)。

摘　　要：目的建立一种QuEchERS-高效液相色谱-串联质谱测定牛奶中6种喹诺酮类兽药残留的方法。方法样品经含1%甲酸的乙腈溶液提取后,用PSA粉末净化,经C18色谱柱分离,三重四级杆质谱检测。结果6种喹诺酮在浓度2.0~100.0 ng/mL范围内线性良好,相关系数>0.999,检出限范围为0.222~0.439μg/kg,定量限范围为0.739~1.464μg/kg,加标回收率为76.27%~97.84%,相对标准偏差为3.22%~8.77%。结论该方法操作简便、快速、准确,可作为牛奶中喹诺酮药物残留的定量检测方法。Objective To develop a methodfor the determination of six quinolones residues in milk by QuEchERS-high-performance liquid chromatography-tandem mass spectrometry.Methods The sample was extracted with acetonitrile containing 1%formic acid,purified with PSA powder,separated by C18 column,and detected by triple quadrupole mass spectrometry.Results The six quinolones showed good linearity in the concentration range of 2.0-100.0 ng/mL,the correlation coefficients were>0.999,the detection limits were 0.222-0.439μg/kg,the quantitative limits were 0.739-1.464μg/kg,the recoveries were 76.27%-97.84%,and the relative standard deviations were 3.22%-8.77%.Conclusion The method is simple,rapid and accurate,and can be used for the quantitative determination of quinolone residues in milk.

关 键 词：牛奶 喹诺酮 QUECHERS 高效液相色谱-串联质谱法 

分 类 号：R115[医药卫生—公共卫生与预防医学]