FGF2对矿化诱导下STAT3介导的h DPSCs成牙分化作用研究  

Effects of FGF2 on odontogenic differentiation of human dental pulp stem cells with induced mineralization via STAT3 pathway

作　　者：王晔[1] 于淼[1] 魏朝[1] 张丞 马永平[1] WANG Ye;YU Miao;WEI Chao;ZHANG Cheng;MA Yong-ping(Department of Stomatology,Baoding Second Hospital,Baoding 071000,China)

机构地区：[1]保定市第二医院口腔科,071000

出　　处：《中华老年口腔医学杂志》2023年第2期65-71,共7页Chinese Journal of Geriatric Dentistry

基　　金：2020年度河北省医学科学研究课题计划(20200170)。

摘　　要：目的探讨成纤维细胞生长因子2(FGF2)对人牙髓干细胞(hDPSCs)成牙分化的影响及对信号转导子与激活子3(STAT3)通路的调节作用。方法hDPSCs随机分为对照组、FGF2组、Stattic组和FGF2+Stattic组,细胞进行成骨诱导。FGF2组细胞20 ng/mL FGF2处理,Stattic组细胞4μM Stattic预处理30 min,FGF2+Stattic组细胞4μM Stattic预处理30 min后,20 ng/mL FGF2处理。CCK-8检测细胞活力,qRT-PCR检测牙本质基质蛋白1(DMP-1)和牙本质涎磷蛋白(DSPP)mRNA相对表达量,检测碱性磷酸酶(ALP)活性,茜素红染色观察矿化情况,蛋白印迹法检测FGF2、p-STAT3、DMP-1和DSPP蛋白相对表达量。结果与对照组比较,FGF2组细胞增殖活性、ALP活性、DMP-1和DSPP mRNA表达量、FGF2、p-STAT3、DMP-1和DSPP蛋白表达量升高(P<0.05);与对照组比较,Stattic组细胞增殖活性和ALP活性减弱、DMP-1和DSPP mRNA表达量以及FGF2、p-STAT3、DMP-1和DSPP蛋白表达量降低(P<0.05);与FGF2组比较,FGF2+Stattic组细胞增殖活性和ALP活性减弱,DMP-1和DSPP mRNA和蛋白相对表达量、FGF2和p-STAT3蛋白相对表达量降低(P<0.05);与Stattic组比较,FGF2+Stattic组细胞增殖活性和ALP活性增强,DMP-1和DSPP mRNA和蛋白相对表达量、FGF2和p-STAT3蛋白相对表达量升高(P<0.05)。结论FGF2可诱导hDPSCs成牙本质分化,可能是通过调节STAT3信号通路发挥作用。Objective To investigate the effect of fibroblast growth factor 2(FGF2)on odontogenic differentiation of human dental pulp stem cells(hDPSCs)with induced mineralization,and the regulation of signal transduction and transcriptional activator 3(STAT3)pathways.Methods hDPSCs were randomly divided into control group,FGF2 group,Stattic group and FGF2+Stattic group,odontogenic differentiation was induced.The FGF2 group was cultured with 20 ng/mL FGF2,the Stattic group was pretreated with 4μM Stattic for 30 min,and the FGF2+Stattic group was pretreated with 4μM Stattic for 30 min,then was cultured with 20 ng/mL FGF2.The proliferation activity was detected by CCK-8.The mRNA expression of dentin matrix protein 1(DMP1)and dentin sialophospho protein(DSPP)was measured by qRT-PCR.Alkaline phosphatase(ALP)activity and alizarin red staining was used to observe mineralization.The relative expressions of FGF2,p-STAT3,DMP-1 and DSPP were detected by western blotting.Results Compared with those in control group,the proliferation activity,ALP activity,mRNA levels of DMP-1 and DSPP,protein levels of FGF2,p-STAT3,DMP-1 and DSPP were increased in FGF2 group(P<0.05);Compared with control group,the proliferation activity,ALP activity,mRNA levels of DMP-1 and DSPP,protein levels of FGF2,p-STAT3,DMP-1 and DSPP were decreased in Stattic group(P<0.05);Compared with FGF2 group,the proliferation activity,ALP activity,mRNA levels of DMP-1 and DSPP,protein levels of FGF2,p-STAT3,DMP-1 and DSPP were decreased in FGF2+Stattic group(P<0.05).Compared with Stattic group,the proliferation activity,ALP activity,mRNA levels of DMP-1 and DSPP,protein levels of FGF2,p-STAT3,DMP-1 and DSPP were increased in FGF2+Stattic group(P<0.05).Conclusion FGF2 can induce odontogenic differentiation of hDPSCs,possibly by regulating STAT3 signaling pathway.

关 键 词：牙髓干细胞成牙本质分化 成纤维细胞生长因子2 信号转导子和转录激活子3 

分 类 号：R780.2[医药卫生—口腔医学]