局部应用增生平水相提取物对实验性口腔癌预防作用的研究  

Study on chemoprophylaxis of experimental oral carcinoma by local application of hyperplastic aqueous extracts from Gopher buccal mucosa

作　　者：张红利 马兆峰[1] 王嘉祺 孙正 关晓兵[2] ZHANG Hong-li;MA Zhao-feng;WANG Jia-qi;SUN Zheng;GUAN Xiao-bing(Department of Stomatology,Shunyi District Hospital,Beijing 101300,China)

机构地区：[1]北京市顺义区医院口腔科,101300 [2]首都医科大学附属北京口腔医院黏膜科,100050

出　　处：《中华老年口腔医学杂志》2023年第2期72-77,共6页Chinese Journal of Geriatric Dentistry

基　　金：北京市自然科学基金(7102070)。

摘　　要：目的研究局部应用不同浓度的增生平水相提取物对7,12-dimethylbenz(a)anthracene(DMBA)诱导实验性口腔癌的化学预防作用。方法DMBA(0.5%)局部涂抹于叙利亚金黄地鼠(6~8周龄)颊囊黏膜,每周3次,连续6周,诱导实验性口腔癌的发生。6周后,阳性对照组不再做处理,给药组以低、中、高三种剂量(0.35g/100ml、0.7g/100ml、1.4g/100ml)增生平水相提取物继续局部涂抹于地鼠颊囊,每周3次,24周处死动物,观察并记录肿瘤的数目和大小,完成组织病理检查,检测细胞增殖指数以及B淋巴细胞瘤-2基因(B-cell lymphoma-2,Bcl-2)蛋白的表达。结果中剂量组和高剂量组的肉眼肿瘤发生率由88.9%(阳性对照组)分别下降为83.3%和78.9%。病理结果显示高剂量组显著降低了上皮单纯增生病灶数目(P<0.05);低、中、高剂量组显著降低了轻-中异常增生的病灶数目(P<0.05)。低、中、高剂量组Brdu增殖指数在癌旁正常黏膜和轻-中度异常增生和浸润癌中均有显著性差异(P<0.05)。Bcl-2蛋白表达(western blotting)检测结果显示:中、高剂量组与阳性对照组相比均有显著性差异(P<0.05,P<0.01)。结论局部应用增生平水相提取物能够通过抑制细胞增殖、促进细胞凋亡的方式阻断实验性口腔癌的发生、发展,并且这种化学预防效果呈一定的剂量依赖性。Objective To investigate the chemoprophylaxis of 7,12-dimethylbenz(a)anthracene(DMBA)induced experimental oral cancer by local application of hyperplastic aqueous extracts in different concentration.Methods DMBA(0.5%)was applied topically to the buccal pouches of Syrian golden hamsters(6~8 weeks old)three times per week for six weeks to induce the development of oral tumors.The positive control group was no longer treated.ZSP water fraction was applied topically to the oral tumor lesions with three doses(0.35g/100ml,0.7g/100ml,and 1.4g/100ml)for later 18 weeks,three times per week and the animals were killed at 24 weeks.We observed the number and size changes of tumors,and detected histopathological examination,Brdu index and Bcl-2 protein expression.Results The incidence of tumor in the medium-dose group and high-dose group decreased from 88.9%(positive control group)to 83.3%and 78.9%respectively.Pathological results showed that high-dose groups significantly reduced the number of simple hyperplasia lesions(P<0.05);The experimental group significantly reduced the number of lesions with mild-moderate dysplasia(P<0.05);There were significant differences in Brdu proliferation index among normal mucosa adjacent to cancer,mild-moderate dysplasia and invasive carcinoma in low,medium and high dose groups(P<0.05).The expression of Bcl-2 protein(Western blotting)showed that the medium and high dose groups had significant differences compared with the positive control group(P<0.05,P<0.01).Conclusions Local application of ZSP water fraction can block the occurrence and development of experimental oral cancer by inhibiting cell proliferation and promoting cell apoptosis,especially in the early stage of oral cancer,and this chemoprophylaxis effect is dose-dependent.

关 键 词：增生平 水相提取物 口腔癌 预防作用 

分 类 号：R781.4[医药卫生—口腔医学]