Exogenous melatonin improves the chilling tolerance and preharvest fruit shelf life in eggplant by affecting ROS-and senescence-related processes  

作　　者：Lijun Song Zhuo Tan Weiwei Zhang Qiang Li Zhaoxiang Jiang Shuxing Shen Shuangxia Luo Xueping Chen 

机构地区：[1]State Key Laboratory of North China Crop Improvement and Regulation,Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei,Collaborative Innovation Center of Vegetable Industry in Hebei,College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China [2]College of Life Sciences,Hebei Agricultural University,Baoding,Hebei 071000,China

出　　处：《Horticultural Plant Journal》2023年第3期523-540,共18页园艺学报（英文版）

基　　金：Introduction of Talents for Scientific Research of State Key Laboratory of North China Crop Improvement and Regulation (Grant No.NCCIR2020RC-11);Hebei Fruit Vegetables Seed Industry Science and Technology Innovation Team Project (Grant No.21326309D);Vegetable Innovation Team Project of Hebei Modern Agricultural Industrial Technology System (Grant No.HBCT2018030203);Introduction of Talents for Scientific Research of Hebei Agriculture University (Grant No.YJ2020048);Basic Scientific Research Funds of Provincial Universities of Hebei Province (Grant No.KY2021056) for the provision of funds。

摘　　要：Low temperature is the most common abiotic stress factors during the eggplant cultivation in solar greenhouses.Melatonin plays important roles in plant resistance to low temperature.However,the role of melatonin in regulating chilling tolerance and extending the preharvest shelf life of eggplant fruits is still unknown.In this study,we investigated the effects of exogenous melatonin on eggplant plants and fruits in response to low temperature.Under simulated low-temperature conditions,exogenous melatonin significantly relieved the chilling symptoms of seedlings by reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels and relative leakage rates.These reductions were caused by higher superoxide dismutase (SOD) and catalase (CAT) activities and increased endogenous polyamine and melatonin levels compared with those in untreated seedlings.Notably,the expression levels of SOD,CAT1/2,and polyamine synthesis genes (ADC and ODC) were also increased by 100μmol·L~(-1)melatonin,as well as those of genes involved in melatonin synthesis (TDC,T5H,SNAT,ASMT,and COMT) and cold regulation (COR1,CBFa/b,and ZAT2/6/12).To further investigate the effects of melatonin on eggplant leaves and fruits under natural low temperature conditions,100μmol·L~(-1)melatonin was sprayed on the functional leaves at three days before commodity maturation.Melatonin significantly alleviated chilling injury in the leaves and pericarp and extended the preharvest shelf life of the fruit by increasing the expression of COR1,CBF,ZAT2/6/12,and API5 and decreasing the expression of senescence-related genes (NCED1/2 and SAG12).Therefore,100μmol·L~(-1)melatonin improved chilling tolerance and fruit shelf life by upregulating ZAT2/6/12 to affect ROS-and senescence-related processes,which provides a reference for alleviating cold stress and extending the preharvest fruit shelf life in eggplant.

关 键 词：EGGPLANT MELATONIN Chilling tolerance Cold regulation Reactive oxygen species Preharvest fruit shelf life 

分 类 号：S641.1[农业科学—蔬菜学]