维生素D及其受体诱导口腔扁平苔藓巨噬细胞IL-10表达  

Vitamin D/VDR Signaling Regulates IL-10 Expression in Macrophages from Oral Lichen Planus

作　　者：葛学军[1,2] 孙卓 赵珍妮 杜杰 GE Xuejun;SUN Zhuo;ZHAO Zhenni;DU Jie(Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials,Shanxi Medical University School and Hospital of Stomatology,Taiyuan 030001,Shanxi,China;Department of Endodontics,Shanxi Medical University School and Hospital of Stomatology,Taiyuan 030001,China)

机构地区：[1]山西医科大学口腔医学院·口腔医院,口腔疾病防治与新材料山西省重点实验室,山西太原030001 [2]山西医科大学口腔医学院·口腔医院,牙体牙髓科,山西太原030001

出　　处：《口腔医学研究》2023年第6期541-546,共6页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81800499)。

摘　　要：目的:探讨维生素D及其受体(VD/VDR)对口腔扁平苔藓(OLP)巨噬细胞炎性因子的调控作用。方法:选取OLP患者和健康对照志愿者各20例,分选组织巨噬细胞检测炎性因子和VDR表达量并分析两者间相关性;在单核源巨噬细胞和THP-1细胞系过表达VDR或加入维生素D,检测细胞白细胞介素-10(IL-10)表达量;染色体免疫共沉淀和荧光素酶报告基因实验检测VDR与IL-10基因启动子结合情况;维生素D处理OLP巨噬细胞检测炎性因子表达量。结果:OLP病变组织巨噬细胞VDR表达水平降低,且与炎症抑制因子IL-10表达水平呈正相关;细胞水平上,VD/VDR信号通路明显诱导巨噬细胞IL-10表达;分子机制上,VDR通过与IL-10基因启动子区域作用元件相结合,激活IL-10基因转录水平;维生素D处理后OLP巨噬细胞IL-10表达上调且促炎因子表达下调。结论:OLP巨噬细胞VD/VDR信号通路通过调控IL-10转录水平影响炎症因子表达。Objective:To investigate the regulatory roles of vitamin D and its receptor VDR in the cytokines production in macrophages from oral lichen planus(OLP).Methods:Twenty OLP patients and 20 healthy volunteers were recruited.Macrophages from oral tissues were isolated,and the expressions of cytokines and VDR was detected.IL-10 levels were measured in monocyte-derived macrophages and THP-1 cells overexpressed VDR or treated with vitamin D.The binding of VDR and its element in the promoter region of IL-10 gene was confirmed by ChIP and luciferase report assays.Cytokines levels were detected in OLP macrophages with vitamin D treatment.Results:VDR expression was decreased in OLP macrophages and positively correlated with IL-10 expression.Vitamin D/VDR signaling up-regulated IL-10 levels in monocyte-derived macrophages and THP-1 cells.Mechanistically,VDR was able to interact with its element in the promoter to enhance the transcription of IL-10.Vitamin D increased IL-10 levels and decreased pro-inflammatory cytokines expression in OLP macrophages.Conclusion:Vitamin D/VDR signaling regulates IL-10 transcription to mediate cytokines levels in OLP macrophages.

关 键 词：口腔扁平苔藓 维生素D 维生素D受体 炎性因子 白细胞介素-10 

分 类 号：R781.5[医药卫生—口腔医学]