对比血清学鉴定与全面生化反应在沙门氏菌检验中的应用分析  

Comparative Serological Identification with Comprehensive Biochemical Reaction in Salmonella Testing

作　　者：曹原[1] Cao Yuan(Department of Health,Kaifeng Center for Disease Control and Prevention,Kaifeng 475002,Henan Province,China)

机构地区：[1]开封市疾病预防控制中心学校卫生科,河南开封475002

出　　处：《罕少疾病杂志》2023年第6期96-97,共2页Journal of Rare and Uncommon Diseases

摘　　要：目的分析在沙门氏菌检验中用血清学鉴定与全面生化反应的应用价值。方法将开封某医院近两年(2021.1~2022.12)收治的沙门氏菌感染者30例作为本次观察对象,在经酶联免疫吸附试验测定后确定为沙门氏菌感染,另将同一时期接收的健康志愿者30例作为对照组观察对象,均采取血清学鉴定与全面生化反应,比较分析在不同检验方式下的准确性(灵敏度、特异度、阳性预测值、阴性预测值)。结果血清学鉴定准确性(灵敏度、特异度、阳性预测值、阴性预测值)更高于全面生化反应,但两种联合检验方式更高于单项检验,对比有统计学意义(P<0.05)。结论在沙门氏菌检验过程中,血清学检验的诊断价值略高于全面生化反应,而联合检验则更有利于提高疾病诊断的灵敏度与特异度,对此,临床应根据患者实际情况选择更合理的检验方案。Objective To analyze the application value of serological identification and comprehensive biochemical reaction in Salmonella test.Methods A total of 30 cases of Salmonella infection admitted to a hospital in Kaifeng in the past two years(2021.1-2022.12)were used as the observation objects,and after determination by enzyme-linked immunosorbent assay,30 cases of healthy volunteers received during the same period were used as observation objects in the control group,all of which were evaluated by serological identification and comprehensive biochemical reaction,and the accuracy(sensitivity,specificity,positive predictive value,negative predictive value)under different test methods was compared and analyzed.Results The accuracy of serological identification(sensitivity,specificity,positive predictive value,negative predictive value)was higher than that of comprehensive biochemical reaction,but the two combined tests were higher than the single test,and the comparison was statistically significant(P<0.05).Conclusion In the process of Salmonella detection,the diagnostic value of serological test is slightly higher than that of comprehensive biochemical reaction,while the combined test is more conducive to improving the sensitivity and specificity of disease diagnosis.

关 键 词：血清学鉴定 全面生化反应 沙门氏菌 检验 

分 类 号：R378.22[医药卫生—病原生物学]