UHPLC-Q-TOF-MS/MS分析青钱柳嫩叶渗漉提取液化学成分  

Analysis of Chemical Constituents in Percolate the Extract of Cyclocarya paliurus Tender Leaves by UHPLC-Q-TOF-MS/MS

作　　者：马博稷 肖岩 陈祖德 舒任庚 李冰涛[1,3] 姜丽 徐国良[1,3,4] 张启云 MA Boji;XIAO Yan;CHEN Zude;SHU Rengeng;LI Bingtao;JIANG Li;XU Guoliang;ZHANG Qiyun(Research Center for Differentiation and Development of Traditional Chinese Medicine(TCM)Basic Theory,Jiangxi University of Chinese Medicine,Nanchang 330004,China;School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Jiangxi Provincial Key Laboratory of TCM Etiopathogenisis,Nanchang 330004,China;Key Laboratory of Pharmacology of Traditional Chinese Medicine in Jiangxi,Nanchang 330004,China)

机构地区：[1]江西中医药大学中医基础理论分化发展研究中心,江西南昌330004 [2]江西中医药大学药学院,江西南昌330004 [3]江西省中医病因生物学重点实验室,江西南昌330004 [4]江西省中药药理学重点实验室,江西南昌330004

出　　处：《食品工业科技》2023年第13期281-291,共11页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(82060826);江西省教育厅科学技术研究项目(GJJ190645);江西中医药大学博士启动基金(2018WBZR008);江西中医药大学校级科技创新团队发展计划(CXTD22015)。

摘　　要：为分析青钱柳嫩叶的化学成分,本研究采取高效液相色谱-四级杆飞行时间串联质谱法(UHPLC-Q-TOFMS/MS)对青钱柳嫩叶渗漉提取液的化学成分进行快速定性分析。在正、负离子模式下全扫描和对母离子电子轰击,根据各化合物的精确分子量及元素组成、主要碎片信息、保留时间以及结合青钱柳相关文献和数据库检索,从青钱柳中共鉴定出94种化合物,包括29种黄酮类、16种三萜类、25种有机酸类、24种其他类化合物。分析结果显示,青钱柳渗漉提取液中含有黄酮、有机酸、三萜、香豆素、氨基酸类等多种化合物,且苹果酸、橙皮素、松脂素、6,7-二羟基香豆素、6-甲基香豆素、5,7-二甲氧基香豆素为首次在青钱柳中发现,本研究结果可为进一步明确青钱柳药效物质基础奠定了基础,为开发青钱柳相关产品提供了理论依据。To analyze the chemical components of the tender leaves of Cyclocarya paliurus,a rapid qualitative analysis of the chemical components of the percolation extracts of the tender leaves of Cyclocarya paliurus was carried out by ultrahigh-performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry(UHPLC-Q-TOF-MS/MS).Mass spectrum data were collected in the positive and negative ion modes,and MassHunter qualitative analysis software was used,according to the retention time and mass spectrum information of each compound,combined with existing literature and mass spectrum databases,the compounds were analyzed for the fragmentation rule,94 compounds were identified from Cyclocarya paliurus,including 29 flavonoids,16 triterpenes,25 organic acids,24 other compounds.The analysis results showed that the percolation extract of Cyclocarya paliurus contained flavonoids,organic acids,triterpenoids,coumarins,amino acids,and other compounds,and malic acid,hesperidin,rosin,6,7-dihydroxycoumarin,6-methylcoumarin,5,7-dimethoxycoumarin are found in Cyclocarya paliurus for the first time.This study can lay a foundation for further clarifying the material basis of Cyclocarya paliurus.It provides a theoretical basis for the development of relevant products of Cyclocarya paliurus.

关 键 词：青钱柳嫩叶 渗漉 化学成分 高效液相色谱-四级杆飞行时间串联质谱法(UHPLC-Q-TOF-MS/MS) 

分 类 号：O657.63[理学—分析化学]