超声波辅助提取美人蕉叶总黄酮工艺及其抗氧化活性研究  

Ultrasonic-assisted Extraction of Total Flavonoids from Canna IndicaLeaves and Its Antioxidant Activity

作　　者：陈文娟[1] 陈建福[2,3] 张铭霞 庄远红 CHEN Wenjuan;CHEN Jianfu;ZHANG Mingxia;ZHUANG Yuanhong(Department of Food Engineering,Zhangzhou City University,Zhangzhou 363000,China;College of Chemical Engineering,Zhangzhou Institute of Technology,Zhangzhou 363000,China;Collaborative Innovation Center of Fine Chemicals in Fujian Province,Zhangzhou 363000,China;Department of Biological Science and Technology,Minnan Normal University,Zhangzhou 363000,China)

机构地区：[1]漳州城市职业学院食品工程系,福建漳州363000 [2]漳州职业技术学院石油化工学院,福建漳州363000 [3]福建省精细化工应用技术协同创新中心,福建漳州363000 [4]闽南师范大学生物科学与技术学院,福建漳州363000

出　　处：《湖北民族大学学报（自然科学版）》2023年第2期147-155,共9页Journal of Hubei Minzu University:Natural Science Edition

基　　金：漳州市自然科学基金项目(ZZ2021J27);福建省中青年教师教育科研项目(JAT210934)。

摘　　要：为开发利用美人蕉资源,探究超声波对美人蕉叶总黄酮提取率的影响,采用响应面法优化了提取工艺,并评价了总黄酮的抗氧化活性。先通过单因素实验考察超声时间、超声温度、液料比和乙醇体积分数对美人蕉叶总黄酮提取率的影响,确定4个单因素的参数范围,再通过Design Expert 8.0.6进行4因素3水平的响应面设计及优化,最后通过美人蕉叶总黄酮对O_(2)^(-)、OH、DPPH自由基的清除能力确定了其抗氧化活性。结果表明,当超声时间为48 min、超声温度为66℃、液料比为61 mL/g和乙醇体积分数为75%时,美人蕉叶总黄酮的提取率最高,为37.53 mg/g,与预测值的相对误差为0.79%,表明该实验模型合理,适合于美人蕉叶总黄酮提取工艺的优化。美人蕉叶总黄酮对O_(2)^(-)、OH、DPPH自由基均表现出较强的清除能力,其IC_(50)值分别为423.90、203.40、78.28 mg/L。该研究为具生物活性的美人蕉叶总黄酮的开发与利用提供方向。In order to develop and utilize Canna indica resources,the effect of ultrasound on the extraction yield of total flavonoids from Canna indica leaves was explored.The extraction process was optimized by response surface methodology,and the antioxidant activity of total flavonoids was evaluated.Firstly,the effects of ultrasonic time,ultrasonic temperature,liquid-to-material ratio and ethanol concentration on the extraction yield of total flavonoids from Canna indica leaves were investigated by single factor,and the parameter ranges of four single factors were determined.Then,the response surface methodology with four factors and three levels was designed and optimized by Design Expert 8.0.6.Finally,the antioxidant activity of total flavonoids from Canna indica leaves was determined by its scavenging ability to O_(2)^(-),OH and DPPH free radica.The results showed that when the extraction time was 48 min,the ultrasonic temperature was 66℃,the liquid-to-material ratio was 61 mL/g and the ethanol concentration was 75%,the extraction yield of total flavonoids in Canna indica leaves was the highest,which was 37.53 mg/g,and the relative error with the predicted value was 0.79%,indicating that the experimental model was reasonable and suitable for the optimization of the extraction process of Canna indica leaves total flavonoids.The total flavonoids of Canna indica leaves showed strong scavenging ability to O_(2)^(-),OH,DPPH free radica,and their IC_(50) values were 423.90,203.40,78.28 mg/L,respectively.This research provided directions for the development and utilization of bioactive Canna indica leaves total flavonoids.

关 键 词：超声波辅助提取 总黄酮 美人蕉叶 响应面法 优化 抗氧化活性 

分 类 号：O629[理学—有机化学] S682[理学—化学] TS202[农业科学—观赏园艺]