鱼藤素通过调控miR-520a-3p影响卵巢癌SKOV3细胞的增殖和凋亡  

Effects of deguelin on the proliferation and apoptosis of ovarian cancer SKOV3 cells by regulating miR-520a-3p

作　　者：莫蕊 陈燕娥[1] 吴学明[1] MO Rui;CHEN Yan'e;WU Xueming(Department of Obstetrics and Gynecology,Haikou Hospital of Maternal and Child Health,Haikou 570203,Hainan,China)

机构地区：[1]海口市妇幼保健院妇产科,海南海口570203

出　　处：《中国肿瘤生物治疗杂志》2023年第5期387-392,共6页Chinese Journal of Cancer Biotherapy

摘　　要：目的:探讨鱼藤素通过调控miR-520a-3p表达对卵巢癌SKOV3细胞增殖和凋亡的影响。方法:将SKOV3细胞分为对照组(鱼藤素0μmol/L)、鱼藤素低剂量(5μmol/L)、中剂量(10μmol/L)、高剂量(20μmol/L)组,miR-NC组、过表达miR-520a-3p组,鱼藤素+anti-miR-NC组、鱼藤素+anti-miR-520a-3p组。CCK-8法、细胞集落形成实验、FCM以及qPCR法分别检测SKOV3细胞的增殖抑制率、细胞克隆形成数、凋亡率以及miR-520a-3p表达水平。结果:与对照组比较,鱼藤素(低、中、高剂量)组SKOV3细胞增殖抑制率、凋亡率、miR-520a-3p表达水平均显著升高(均P<0.05),细胞克隆形成数显著减少(P<0.05)。与miR-NC组比较,过表达miR-520a-3p组SKOV3细胞的增殖抑制率、凋亡率均显著升高(均P<0.05),细胞克隆形成数显著减少(P<0.05)。与鱼藤素+anti-miR-NC组比较,鱼藤素+anti-miR-520a-3p组SKOV3细胞的增殖抑制率、凋亡率均显著降低(均P<0.05),细胞克隆形成数显著增多(P<0.05)。结论:鱼藤素通过增加miR-520a-3p表达抑制卵巢癌SKOV3细胞的增殖能力,并诱导其凋亡。Objective:To investigate the effects of deguelin on the proliferation and apoptosis of ovarian cancer SKOV3 cells by regulating miR-520a-3p.Methods:The SKOV3 cells were divided into the control group(deguelin 0μmol/L),low-dose deguelin group(5μmol/L),medium-dose deguelin group(10μmol/L),and high-dose deguelin group(20μmol/L),miR-NC group,miR-520a-3p group,deguelin+anti-miR-NC group and deguelin+anti-miR-520a-3p group.CCK-8,colony formation experiment,flow cytometry and qPCR were used to detect the inhibition rate,the number of clone formation,the apoptosis rate and the miR-520a-3p expression of SKOV3 cells respectively.Results:Compared with the control group,the inhibition rate,the apoptosis rate,miR-520a-3p expression of SKOV3 cells in the deguelin(low,medium,and high dose)groups were significantly increased(all P<0.05),and the number of clone formation was significantly reduced(P<0.05).Compared with the miR-NC group,the inhibitory rate and the apoptosis rate of SKOV3 cells in the miR-520a-3p group were significantly increased(all P<0.05),and the number of clone formation was significantly reduced(P<0.05).Compared with the deguelin+anti-miR-NC group,the inhibition rate and the apoptosis rate of SKOV3 cells in the deguelin+anti-miR-520a-3p group were significantly reduced(all P<0.05),and the number of clone formation was significantly increased(P<0.05).Conclusion:Deguelin inhibited the proliferation potential of ovarian cancer SKOV3 cells and induced cell apoptosis by increasing the expression of miR-520a-3p.

关 键 词：卵巢癌 SKOV3细胞 鱼藤素 miR-520a-3p 细胞增殖 凋亡 

分 类 号：R737.31[医药卫生—肿瘤] R730.52[医药卫生—临床医学]