基于气相色谱-质谱联用技术的甲状腺功能亢进性心脏病大鼠心脏组织代谢组学分析  

Metabonomic analysis of myocardial tissues in rat models of hyperthyroid heart based on GC-MC

作　　者：董靖[1] 张丽 熊红丽 朱士胜[3] 陈燕梅[4] DONG Jing;ZHANG Li;XIONG Hongli;ZHU Shisheng;CHEN Yanmei(Department of Clinical Medicine,Chongqing Medical and Pharmaceutical College,Chongqing 401331,China;Department of Basic Medicine,Chongqing College of Traditional Chinese Medicine,Chongqing 402760,China;Department of Basic Medical Sciences,Chongqing Medical and Pharmaceutical College,Chongqing 401331,China;Department of Endocrinology,Yongchuan hospital of Chongqing Medical University,Chongqing 402160,China)

机构地区：[1]重庆医药高等专科学校临床医学院,401331 [2]重庆中医药学院基础部,402760 [3]重庆医药高等专科学校基础部,401331 [4]重庆医科大学附属永川医院内分泌科,402160

出　　处：《重庆医学》2023年第11期1620-1626,1632,共8页Chongqing medicine

基　　金：重庆市自然科学基金面上项目(cstc2019jcyj-msxmX0455);重庆市教委科学技术研究项目(KJQN201902804,KJQN201902805);重庆医药高等专科学校创新研究群体项目(YGZ2019402);重庆市科卫联合医学科研项目(2019MSXM033,2023MSXM074);重庆医药高等专科学校校级自然科学重点项目(ygz2020102,ygz2021119);重庆医药高等专科学校人才引进项目(ygz2019308)。

摘　　要：目的通过气相色谱-质谱(GC-MS)联用技术探究甲状腺功能亢进性心脏病(HHD)大鼠心肌组织代谢物变化,筛选相关代谢通路,阐明HHD可能的发病机制。方法将20只SD大鼠随机分为对照组和HHD组,给予相应药物干预后,采集大鼠心脏组织进行GC-MS分析,结合多变量、单变量统计分析及数据库检索,筛选出HHD可能的潜在生物标志物,利用MetaboAnalyst 5.0软件对筛选出的潜在生物标志物进行代谢通路分析。结果相对于对照组,HHD组共筛选鉴定出57种差异代谢物作为潜在生物标志物,这些潜在生物标志物参与了39条代谢通路,其中丙氨酸、天冬氨酸和谷氨酸代谢,β-丙氨酸代谢,牛磺酸和亚牛磺酸代谢,甘氨酸、丝氨酸和苏氨酸代谢,戊糖和葡萄糖醛酸相互转化,氨酰-tRNA生物合成其6条代谢通路变化明显。结论HHD心脏组织中氨基酸、糖类、脂类和核苷酸等代谢水平发生明显变化;上述6条代谢通路异常可能是HHD潜在发病机制。Objective To investigate the metabolite changes in myocardial tissues of hyperthyroid heart rats by gas chromatography-mass spectrometry(GC-MS),and screen the related metabolic pathways and elucidate the possible pathogenesis of hyperthyroid heart disease(HHD).Methods A total of twenty SD rats were randomly divided into the control group and the HDD group.After administration of corresponding drug intervention,heart tissues were collected for GC-MS analysis,combined with multivariate and univariate statistical analysis and database search to screen out possible potential biomarkers of HHD.MetaboAnalyst 5.0 software was used to perform metabolic pathway analysis.Results A total of 57 differential metabolites were screened and identified as potential biomarkers in the HHD group compared with the control group.These potential biomarkers were mainly involved in 39 metabolic pathways,of which alanine,glutamic acid and aspartic acid metabolism,β-alanine metabolism,taurine and the metabolism of taurine,glycine,serine and threonine metabolism and mutual transformation between the pentose and glucuronic acid and ammonia acyl-tRNA biosynthesis of six changes in metabolic pathwaysshowed significant changes.Conclusion The metabolic levels of amino acids,sugars,lipids and nucleotides were significantly altered in HHD heart tissues.The above six metabolic pathway abnormalities may be the potential pathogenesis of HHD.

关 键 词：甲状腺功能亢进性心脏病 代谢组学 气相色谱-质谱 生物标志物 代谢通路分析 

分 类 号：R581.1[医药卫生—内分泌]