Exploitation of the antibacterial,antibiofilm and antioxidant activities of Salvadora Persica(Miswak)extract  

作　　者：Gamal M.El-Sherbiny Ahmed Mohamed Gazelly Mohammed H.Sharaf Saad A.Moghannemm Shehata M.E Mahmoud K.A.Ismail Ahmad S.El-Hawary 

机构地区：[1]Botany and Microbiology Department,Faculty of Science(Boys),Al-Azhar University,Cairo 11884,Egypt

出　　处：《Journal of Bioresources and Bioproducts》2023年第1期59-65,共7页生物质资源与工程（英文）

摘　　要：The Salvadora persica(S.persica)L.chewing stick,usually known as miswak,is still being em-ployed as an oral hygiene agent for plaque and gingivitis prevention.This study aims to assess the antibacterial,antibiofilm,antioxidant,and phytochemical profile of S.persica extract.The S.per-sica was purchased from a local market,grinded and extracted with petroleum ether.The disk dif-fusion,microdilution,and micro-plate assays were performed to evaluate the antibacterial and an-tibiofilm activities of the prepared extract at different concentrations against𝛽-lactam resistance Streptococcus species.Free radical scavenging 2,2-diphenyl-1-picrylhydrazyl(DPPH)and stable radical cationic chromophore,2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate)(ABTS)methods were used to determine their antioxidant activity.Chromatographic and spectrometric analyses were performed using gas chromatography-mass(GC-MS)spectrometry.The minimum inhibitory concentration(MIC)of S.persica extract against𝛽-lactam resistance Streptococcus species ranged from 6.25 to 12.5 mg/mL.The maximum suppression of biofilm formation by S.persica extract was observed at MIC with a percentage of 68.66%,against Streptococcus oralis.The S.persica ex-tract exhibited antioxidant activity with IC 50 of 20μg/mL and 35μg/mL from DPPH and ABTS,respectively.The phytochemical characterization showed the presence of 22 compounds with ma-jor compounds;benzyl isothiocyanate(36.21%)and n-hexadecanoic acid(27.62%).The S.persica extract exhibited antibacterial activity against𝛽-lactam resistant Streptococcus species,showing a promising natural alternative that could be a treatment option.

关 键 词：β-lactam resistance Streptococcus species S.persica extract ANTIBACTERIAL ANTIBIOFILM ANTIOXIDANT GC-MS analysis 

分 类 号：TS202[轻工技术与工程—食品科学]